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首页> 外文会议>International Conference on Biomedical and Biological Engineering >Leaf Age Promotes Agrobacterium-Mediated Transformation of Hybrid Poplar Populus Davidiana Dode x P. Bollena Lauche
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Leaf Age Promotes Agrobacterium-Mediated Transformation of Hybrid Poplar Populus Davidiana Dode x P. Bollena Lauche

机译：叶龄促进农杆菌介导的杂交杨树杨树DODE X P. Bollena Lauche的转化

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摘要

Poplar as a woody-plant model is significant to improve the transformation efficiency of poplar. The effect of different leaf age as explants on transformation frequency was studied. The results exhibited that the higher transformation frequency was generated by 30-day-old leaf explants, compared with 60-day-old and 90-day-old leaf explants. The transformation frequency was more than 30%. The results indicated that various of transformation frequency were led by different leaf ages explants under the same conditions. The status of Agrobacterium attachment were observed by scanning electron microscopy (SEM) in the process of transformation. The results exhibited that Agrobacterium attachment were gradually reduced with the increased in leaf ages. Leaf surface at thirty-day-old showed massive Agrobacterium attachment. Our results suggest that younger leaves had higher transformation efficiency than older leaves.
机译：杨树作为木本植物模型是提高杨树的转化效率的重要意义。研究了不同叶绿症作为转化频率的外植体的影响。结果表明，与60天老和90天的叶片外植体相比，30天老叶片外植物产生了较高的转化频率。转化频率超过30％。结果表明，在相同条件下，不同的叶子年龄的外植体引用各种转化频率。通过在转化过程中扫描电子显微镜（SEM）观察到农杆菌附着的状态。结果表明，随着叶片的增加，农杆菌附着逐渐降低。在三十天龄的叶表面显示出巨大的土壤杆菌附件。我们的研究结果表明，年轻的叶子比老年人更高的转变效率。

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