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首页> 外文会议>IEEE Healthcare Innovation Point-of-Care Technologies Conference >Characterization of a compact and highly sensitive fluorescence-based detection system for point-of-care applications
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Characterization of a compact and highly sensitive fluorescence-based detection system for point-of-care applications

机译：表征紧凑型高敏感的基于荧光检测系统，用于护理点应用

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摘要

This work details the design and characterization of a low-cost, portable and highly sensitive fluorescence detection system intended for use in a compact and disposable point-of-care (POC) device. The detection device leverages time integration to improve the signal to noise ratio (SNR) compared to instantaneous measurements. It also eliminates complicated focusing optics and electronics typically found in bulky and expensive laboratory-scale devices. Characterization was performed by measuring a series of logarithmically scaled dilutions of 1 μm Nile Red fluorescent microspheres immobilized on microscope slides. This approach eliminates assay dependencies and elucidates the actual system performance. A theoretical model that predicts the time-integrated output voltage profile of the sensor was developed; this prediction is useful for evaluating any lens-free fluorescent system based on a set of filters and choice of fluorophore. By relating the fluorophore concentration, system design parameters, and the output voltage, the model matches well with the empirical data and the limit of determination (LOD) for Nile Red is 20 particles. This system provides a sensitive and potentially low-cost device for fluorescent diagnosis in an integrated and compact/miniaturized POC device, lab-on-chip or a table-top reader.
机译：这项工作详细说明了旨在用于紧凑型和一次性护理点（POC）装置的低成本，便携式和高灵敏度荧光检测系统的设计和表征。与瞬时测量相比，检测装置利用时间积分来改善信噪比（SNR）的信号。它还消除了通常在庞大和昂贵的实验室级设备中发现的复杂聚焦光学和电子产品。通过测量固定在显微镜载玻片上的1μm尼罗红荧光微球的一系列对数上缩放稀释液进行表征。该方法消除了测定依赖性并阐明了实际的系统性能。一种理论模型，可以开发了传感器的时间集成输出电压分布;该预测可用于评估基于一组过滤器和荧光团的选择的无透镜荧光系统。通过涉及荧光团浓度，系统设计参数和输出电压，模型与经验数据匹配良好，尼罗红色的测定极限（LOD）是20个颗粒。该系统在集成和紧凑的/小型化POC设备，芯片片或台式读卡器中提供了一种敏感和潜在的低成本装置，用于荧光诊断，用于芯片片或桌面读卡器。

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