首页>
外国专利>
A NOVEL RAPID AND HIGHLY SENSITIVE CELL BASED SYSTEM FOR THE DETECTION AND CHARACTERIZATION OF HIV
A NOVEL RAPID AND HIGHLY SENSITIVE CELL BASED SYSTEM FOR THE DETECTION AND CHARACTERIZATION OF HIV
展开▼
机译：基于新型快速，高度灵敏细胞的HIV检测和鉴定系统
展开▼
页面导航
摘要
著录项
相似文献
摘要
The invention pertains to a novel cell line, an HIV tat-rev dependent GFP-Gaussia luciferase Reporter cell line, known henceforth as the GGR cell line, that detects pseudotype and replication competent HIV (cloned or uncloned isolates, in cell media or human serum) rapidly and with high sensitivity. This GGR cell line provides an improved method of characterizing the entry phenotype of HIV envelope genes, and detecting and examining primary HIV samples in the context of laboratory research, clinical trial monitoring, and medical diagnostics. Examples include, but are not limited to, determining the functional HIV viral load, responsiveness to treatment, characterization of viral co-receptor usage (testing for viral co-receptor usage, i.e., CCR5 vs CXCR4, as required prior to prescribing FDA-approved CCR5 inhibitors), and characterization of other viral or drug resistance phenotypic properties to guide treatment.
展开▼
机译：本发明涉及一种新颖的细胞系，一种HIV tat-rev依赖性GFP-高斯荧光素酶报告基因细胞系，此后称为GGR细胞系，其在细胞培养基或人血清中检测假型和复制能力的HIV（克隆或未克隆的分离株） ），并且具有很高的灵敏度。该GGR细胞系提供了一种改进的方法，用于表征HIV包膜基因的进入表型，并在实验室研究，临床试验监测和医学诊断的背景下检测和检查主要HIV样品。实例包括但不限于确定功能性HIV病毒载量，对治疗的反应性，病毒共受体使用的表征（在处方FDA批准之前按要求测试病毒共受体使用，即CCR5 vs CXCR4） CCR5抑制剂），并通过其他病毒或药物抗性表型特性进行表征以指导治疗。
展开▼
