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An ESIPT‐Based Ratiometric Fluorescent Probe for Highly Sensitive and Rapid Detection of Sulfite in Living Cells

机译：基于ESIPT的比率荧光探针，用于高度敏感和快速检测活细胞中亚硫酸盐

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The novel ratiometric fluorescent probe HPQRB with an ESIPT effect based on Michael addition for highly sensitive and fast detection of sulfite in living HepG2 cells is reported. HPQRB can be easily synthesized by a two‐step condensation reaction. HPQRB has a large emission shift (Δ λ =116?nm), which is beneficial for fluorescence imaging research, and its sulfite‐responsive site is based on a rhodamine‐like structure with the emission peak at 566?nm, which decreases with increasing sulfite concentration. and its HPQ structure always has an ESIPT effect throughout the reaction process, keeping the emission peak at 450?nm as a self‐reference. In particular, HPQRB has high selectivity for sulfite and responds quickly (within 30?s) with a low detection limit (44?nM). Furthermore, HPQRB has been successfully used for fluorescence imaging of sulfite in HepG2 cells, demonstrating the superior ability to detect sulfite under physiological conditions. Waiting for the sulfite to shine : HPQRB is a ratiometric fluorescent probe that responds quickly (within 30?s) and selectively to sulfite with a low detection limit (44?nM) and it has been successfully used for fluorescence imaging of sulfite in HepG2 cells, demonstrating the superior ability to detect sulfite under physiological conditions.
机译：报道了基于MICHAEL的高度敏感和快速检测亚硫酸盐的迈克尔的eSIPT效应的新型比率荧光探针HPQRB。 HPQRB可以通过两步冷凝反应容易地合成。 HPQRB具有大的发射移位（Δλ= 116℃），这对荧光成像研究有益，其亚硫酸盐响应部位基于罗丹明的结构，其发射峰值在566Ω·Nm下，随着增加而减少亚硫酸盐浓度。其HPQ结构在整个反应过程中始终具有eSipt效应，使排放峰保持在450℃，作为自我参考。特别地，HPQRB对亚硫酸盐具有高选择性，并且在检测极限（44Ω·NM）中快速响应（30？S内）。此外，HPQRB已成功用于HepG2细胞中亚硫酸盐的荧光成像，证明在生理条件下检测亚硫酸盐的优异能力。等待亚硫酸盐闪耀：HPQRB是一种比例荧光探针，其迅速响应（30？s内），并选择性地具有低检测限（44μm）的亚硫酸盐，并且已成功用于Hepg2细胞中亚硫酸盐的荧光成像，展示在生理条件下检测亚硫酸盐的优异能力。

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