Characterization of a compact and highly sensitive fluorescence-based detection system for point-of-care applications

机译：用于即时医疗应用的紧凑型高灵敏度基于荧光的检测系统的表征

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This work details the design and characterization of a low-cost, portable and highly sensitive fluorescence detection system intended for use in a compact and disposable point-of-care (POC) device. The detection device leverages time integration to improve the signal to noise ratio (SNR) compared to instantaneous measurements. It also eliminates complicated focusing optics and electronics typically found in bulky and expensive laboratory-scale devices. Characterization was performed by measuring a series of logarithmically scaled dilutions of 1 μm Nile Red fluorescent microspheres immobilized on microscope slides. This approach eliminates assay dependencies and elucidates the actual system performance. A theoretical model that predicts the time-integrated output voltage profile of the sensor was developed; this prediction is useful for evaluating any lens-free fluorescent system based on a set of filters and choice of fluorophore. By relating the fluorophore concentration, system design parameters, and the output voltage, the model matches well with the empirical data and the limit of determination (LOD) for Nile Red is 20 particles. This system provides a sensitive and potentially low-cost device for fluorescent diagnosis in an integrated and compact/miniaturized POC device, lab-on-chip or a table-top reader.

机译：这项工作详细介绍了旨在用于紧凑型和一次性护理点（POC）设备的低成本，便携式和高灵敏度荧光检测系统的设计和特性。与瞬时测量相比，该检测设备利用时间积分来改善信噪比（SNR）。它还消除了通常在笨重且昂贵的实验室规模的设备中常见的复杂的聚焦光学器件和电子设备。通过测量一系列固定在显微镜载玻片上的1μm尼罗红荧光微球的对数刻度稀释液来进行表征。这种方法消除了测定的依赖性，并阐明了实际的系统性能。建立了一个预测传感器的时间积分输出电压曲线的理论模型。该预测对于评估基于一组滤镜和荧光团的无透镜荧光系统很有用。通过关联荧光团浓度，系统设计参数和输出电压，该模型与经验数据非常匹配，尼罗红的测定极限（LOD）为20个粒子。该系统为集成，紧凑/小型化的POC设备，片上实验室或台式阅读器中的荧光诊断提供了一种灵敏的，潜在的低成本设备。

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来源
《2016 IEEE Healthcare Innovation Point-Of-Care Technologies Conference》|2016年|117-120|共4页
会议地点 Cancun(MX)
作者
Uwadiae Obahiagbon; Dixie Kullman; Joseph T. Smith; Benjamin A. Katchman; Hany Arafa; Karen S. Anderson; Jennifer Blain Christen;
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作者单位

Department of Electrical Engineering, Arizona State University, Tempe, AZ, 85281, USA;

Department of Electrical Engineering, Arizona State University, Tempe, AZ, 85281, USA;

Center for Personalized Diagnostics, BioDesign Institute, Arizona State University, Tempe, AZ, 85281, USA;

Center for Personalized Diagnostics, BioDesign Institute, Arizona State University, Tempe, AZ, 85281, USA;

Department of Electrical Engineering, Arizona State University, Tempe, AZ, 85281, USA;

Center for Personalized Diagnostics, BioDesign Institute, Arizona State University, Tempe, AZ, 85281, USA;

Department of Electrical Engineering, Arizona State University, Tempe, AZ, 85281, USA;

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原文格式 PDF
正文语种 eng
中图分类
关键词
Photodiodes; Voltage measurement; Detectors; Mathematical model; Light emitting diodes; Microscopy; Fluorescence;

机译：光电二极管电压测量检测器数学模型发光二极管显微镜荧光;

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Characterization of a compact and highly sensitive fluorescence-based detection system for point-of-care applications

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