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From food microbiological analysis by culture to the liberation of DNA sequencing and beyond: Developments, challenges and future trends in reference methods and industry standards

机译:从培养中的食物微生物学分析到解放DNA测序和超越:参考方法和行业标准的发展,挑战和未来趋势

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Rapid developments in sequencing technology along with falling cost brought widespread opportunities and changes in food microbiological analysis. Long term established practices and analytical 'gold standards', such as food analysis by culture and microbiological typing with PFGE or MLST, change or could be made redundant. The completion of the human genome draft back in 2000 required a decade of international collaboration and billions of dollars, however, nowadays bacterial genomes can be sequenced in hours for less than $100 with what is collectively termed as 'next generation sequencing' technology. The increasing accessibility to sequencing whole genomes and ability of laboratories to identify, and differentiate bacterial and viral strains, reform the practice and expectations in managing quality and safety in food industry. While next generation sequencing is still shaping, 3rd generation sequencing is in progress and bringing ultimate portability. Further emerging technologies such as user-friendly bioinformatics software tools, next-generation robotics, novel sensor materials and drones, start making their way to food analysis applications. While government authorities and global industries have taken initiative, the majority of food industry lacks awareness and capability to fully understand the benefits, issues and future challenges in the changes of conducting food microbiological analysis. The lecture will attempt a review of practices and methods that underlined the industry microbiology standard up to date, recent developments that change the way of work and coming technological opportunities and challenges that are expected to shape the future.
机译:排序技术的快速发展以及降低成本带来了广泛的机遇和食物微生物分析的变化。长期既定的实践和分析的“黄金标准”,如培养和微生物学用PFGE或MLST的微生物键入粮食分析,改变或者可以冗余。 2000年的人类基因组起草的完成需要十年的国际合作和数十亿美元,但是,如今可以在小时内测序,以额定为“下一代测序”技术。越来越多的对整个基因组和实验室能力识别和分化细菌和病毒菌株的能力,改革了食品工业质量和安全的实践和期望。虽然下一代测序仍在塑造,但是第3个生成测序正在进行并带来最终的可移植性。进一步的新兴技术,如用户友好的生物信息学软件工具,下一代机器人,新型传感器材料和无人机,开始进行食物分析应用。虽然政府当局和全球产业进行了主动性,但大多数食品行业缺乏认识和能力,充分了解导电食物微生物学分析的变化中的利益,问题和未来挑战。该讲座将尝试审查迄今为止强调行业微生物学标准的实践和方法,最近的发展改变了工作方式以及需要塑造未来的技术机遇和挑战。

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