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首页> 外文会议>IMEKOFOODS Conference >P47: EXTRACTION, PURIFICATION AND EVALUATION OF FOOD-GRADE PHYCOCYANIN FROM SPIRULINA PLATENSIS
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P47: EXTRACTION, PURIFICATION AND EVALUATION OF FOOD-GRADE PHYCOCYANIN FROM SPIRULINA PLATENSIS

机译：P47：螺旋藻植物等级植物等级植物植物素的提取，纯化和评价

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摘要

Phycocyanin (C-PC) is the major phycobiliprotein in Spirulina (Arthrospira) platensis (Sp.), which is a cyanobacterium with a massive commercial value representing a nutrient-dense food source. This study describes a simple protocol for purifying C-phycocyanin from Spirulina Sp.. The cell lysis of blue-green algae has been carried out by sonication and repeated cycles of freezing and thawing. Among the various buffers used for phycocyanin yield efficiency, phosphate buffer (pH 7, 0.1 M) was found to be the most suitable for highest yield. The purification process involves a multistep treatment of the crude extract, of purity 1.41, by fractional precipitation with ammonium sulfate followed by dialysis and ion-exchange chromatography on DEAE-Sepharose Fast Flow column obtaining food-grade phycocyanin. The purity of phycocyanobilin chromophore has been tested by UV-Vis spectrophotometry by monitoring the absorption after each stage of purification. Also, separation and identification of the purified protein has been achieved by High Performance Liquid Chromatography with Photodiode-array Detection.
机译：植物蛋白（C-PC）是螺旋藻（Arthrospira）Platchetsis（SP。）的主要Phycobiliprotein，其是一种蓝藻，具有巨大的商业价值，代表营养 - 密集的食物来源。本研究描述了一种简单的方案，用于从螺旋藻SP纯化C-浮蛋白。通过超声处理和重复的冷冻和解冻循环进行蓝绿藻的细胞裂解。在用于植物蛋白产生效率的各种缓冲器中，发现磷酸盐缓冲液（pH7,0.1M）是最适合最高产率的。纯化过程涉及通过用硫酸铵分级沉淀，然后透析和离子交换色谱法在DEAE-Sepharose快速流动柱获得食品级植物蛋白的透析和离子交换色谱，纯化1.41对纯度1.41的粗提物处理。通过UV-Vis分光光度法通过监测每个纯化后的吸收的吸收来测试植物细胞的纯度。此外，通过高性能液相色谱法利用光电二极管阵列检测，实现了纯化蛋白的分离和鉴定。

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