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首页> 外文会议>International Conference of Global Network for Innovative Technology >Measurements of Thermal Conductivity and Thermal Diffusivity of Hen Egg-White Lysozyme Crystals Using a Short Hot Wire Method
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Measurements of Thermal Conductivity and Thermal Diffusivity of Hen Egg-White Lysozyme Crystals Using a Short Hot Wire Method

机译：使用短的热线法测量母鸡蛋白溶菌酶晶体的导热性和热扩散性

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Thermal conductivity and thermal diffusivity of hen egg-white lysozyme (HEWL) crystals were examined by using the transient short hot wire method. This method is based on the conventional hot wire method, but improved by using a wire that is much shorter than conventional ones. The magneto-Archimedes levitation technique was utilized to attach the HEWL crystals onto the wire. Owing to the upward magnetic force, the HEWL crystals were deposited at the air-liquid interface of the protein buffer solution where the short hot wire was preliminarily fixed. In situ observation clarified that the wire was completely buried into the HEWL crystals. By means of these techniques, the measurement of thermal conductivity and thermal diffusivity of HEWL crystals was realized for the first time. Gadolinium chloride (a paramagnetic subject) was used as a precipitant agent of crystallization. Crystal growth was carried out over 20 h at 17.2 °C. The applied magnetic field was 4 T. Measurements were conducted during the crystal growth at two different times. The thermal conductivity and diffusivity of the HEWL crystals were determined to be 0.410 W/(m-K) and 3.77xl0~(-8)m~2/s at 14 h after, and 0.438 W/(m-K) and 5.18xl0~(-8)m~2/s at 20 h after, respectively. We emphasize that this method is versatile and applicable for other protein crystals.
机译：通过使用瞬态短的热线方法检查母鸡蛋白溶菌酶（HENWL）晶体的导热性和热扩散性。该方法基于传统的热线方法，但是通过使用比传统方式短得多的电线改善。利用磁性轴升悬浮技术将HEWL晶体附接到电线上。由于向上磁力，在蛋白质缓冲溶液的空气液体界面处沉积了HEWL晶体，其中短的热线预先固定。原位观察澄清说，电线被完全埋入Hewl晶体中。借助于这些技术，首次实现了HEWL晶体的热导率和热扩散性的测量。使用氯化钆（顺磁对象）作为结晶的沉淀剂。晶体生长在17.2℃下超过20小时进行。所施加的磁场为4吨。在两次不同时间的晶体生长期间进行测量。在14小时后测定HEWL晶体的导热率和扩散性为0.410W /（MK）和3.77×10〜（-8）m〜2 / s，0.438W /（MK）和5.18xL0〜（ - 8）分别为20小时的M〜2 / s。我们强调这种方法是通用的，适用于其他蛋白质晶体。

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