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Bacterial Degradation of Free Cyanide in Alkaline Medium Using Bacillus Licheniformis Strain

机译：芽孢杆菌菌株碱性介质中自由氰化物的细菌降解

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Cyanide is a toxic chemical used in mining industry to recover gold and silver in leaching process. Effluents containing cyanide can be treated by bioremediation instead of physical and chemical processes which are often more expensive and less efficient. This study evaluated the capacity for free cyanide degradation of a Bacillus licheniformis strain previously isolated from an abandoned gold mine. First, bacterial growth in LB medium through OD_(600) was studied. Then, bacteria was adapted to grow on alkaline medium using CAPS buffer to keep pH10. Finally, cyanide degradation assays were performed using a 500 mgL~(-1) KCN pH10 50 mM CAPS solution. Free cyanide was measured using a polarographic method. Temperature influence was evaluated at 27, 32 and 37°C. Results showed that B. licheniformis was able to degrade 99% of free cyanide after 5 days of incubation and works better at 32°C showing the strain potential for cyanide biotreatment in mining industry.
机译：氰化物是采矿业中使用的有毒化学品，以恢复浸出过程中的金银。含氰化物的污水可以通过生物化而不是物理和化学方法处理，这些方法通常更昂贵，效率较低。该研究评估了先前从废弃金矿中分离的芽孢杆菌的氰化物降解的氰化物降解的能力。首先，研究了LB培养基中的细菌生长，研究了OD_（600）。然后，使用帽缓冲液使细菌在碱性培养基上生长以保持pH10。最后，使用500mgL〜（-1）KCN pH10 50mM帽溶液进行氰化物降解测定。使用极谱法测量自由氰化物。在27,32和37℃下评估温度影响。结果表明，B.Hicheniformis能在孵育5天后能够降解99％的自由氰化物，在32°C下工作，显示在采矿工业中氰化物生物处理的菌株潜力。

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