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首页> 外文期刊>Proceedings of the Zoological Society >Optimization of Culture Conditions for Production of Protease by Two Bacterial Strains, Bacillus licheniformis BF2 and Bacillus subtilis BH4 Isolated from the Digestive Tract of Bata, Labeo bata(Hamilton)
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Optimization of Culture Conditions for Production of Protease by Two Bacterial Strains, Bacillus licheniformis BF2 and Bacillus subtilis BH4 Isolated from the Digestive Tract of Bata, Labeo bata(Hamilton)

机译：从巴塔虫消化道分离的两个细菌菌株地衣芽孢杆菌BF2和枯草芽孢杆菌BH4生产蛋白酶的培养条件的优化

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摘要

The culture conditions for extracellular protease production by two bacterial strains, Bacillus licheniformis BF2 and Bacillus subtilis BH4 isolated from the foregut and hindgut regions, respectively, of bata(Labeo bata, Cyprinidae)were optimized to obtain maximum level of protease. Both strains were cultured in TSA broth for 24 h at 37 ± 2 °C. This was used as the inoculum for the production medium. The fermentation medium was seeded with 1.0, 2.0, 3.0, 4.0, and 5.0 % inoculum(TSA)and incubated in static culture at 37 °C to standardize the inoculum size for fermentation. The effects of fermentation condition, moisture, pH, temperature, inoculum size, and nitrogen sources on protease production by the isolated bacterial strain were studied. The strain, BH4, produced more protease in comparison to the strain BF2 isolated from the same fish in solid-state fermentation. Enzyme production in both the isolates increased in an optimum pH range of 6.0-6.5. Maximum protease production by both the strains was obtained at 40 °C. To standardize the fermentation period for protease production, production rate was measured at 12-h intervals up to 120 h. Enzyme production increased for 72 h of fermentation in both strains, and decreased thereafter. The enzyme production increased with increased inoculum size up to 3.0 percentage points. Beef extract as the nitrogen source was most effective in optimizing enzyme production by both the isolated strains. The information generated from the present investigation will help to standardize the requirements for optimum production of protease by protease-producing fish gut bacteria.
机译：优化了分别从巴塔（Labeo bata，Cyprinidae）的前肠和后肠区域分离的两个细菌菌株地衣芽孢杆菌BF2和枯草芽孢杆菌BH4产生细胞外蛋白酶的培养条件，以获得最大水平的蛋白酶。两种菌株均在TSA肉汤中于37±2°C下培养24 h。将其用作生产培养基的接种物。发酵培养基上接种有1.0％，2.0％，3.0％，4.0％和5.0％的接种物（TSA），并在37°C的静态培养中孵育以标准化用于接种的接种物大小。研究了发酵条件，水分，pH，温度，接种量和氮源对分离的细菌菌株产生蛋白酶的影响。与在固态发酵中从同一条鱼中分离出的BF2菌株相比，BH4菌株产生了更多的蛋白酶。在6.0-6.5的最佳pH范围内，两种分离物中的酶产生均增加。在40℃下获得了两种菌株的最大蛋白酶生产。为了标准化蛋白酶生产的发酵时间，以12小时为间隔，直至120小时测量了生产速率。在两种菌株中发酵72小时，酶的产量均增加，此后降低。随着接种量的增加，酶的产量增加了3.0个百分点。牛肉提取物作为氮源在优化分离的两种菌株的酶生产方面最有效。从本次调查中获得的信息将有助于标准化产生蛋白酶的鱼肠细菌对最佳蛋白酶生产的要求。

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