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首页> 外文会议>Recent Advances in Fundamental and Applied Sciences Conference >In Vitro Antimicrobial Activity of Extracts and Isolated Compound from Dalbergia stipulacea Roxb. Leaves
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In Vitro Antimicrobial Activity of Extracts and Isolated Compound from Dalbergia stipulacea Roxb. Leaves

机译：来自达伯利亚罗克斯罗克卜提取物和分离化合物的体外抗微生物活性。树叶

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摘要

The study was designed to examine the in vitro antimicrobial efficacy of extracts and isolated compound of Dalbergia stipulacea. Combined extracts (chloroform and methanol) of plant leaves fractionated with n-butanol loaded with column afforded a flavonoid glycoside compound identified as luteolin 4'-rutinoside. Different extracts and isolated compound exhibited pronounced antibacterial and antifungal varied activities against four bacteria (Clostridium acetobutylinium, Bacillus subtilis, Streptococcus mutans, and Pseudomonas sp.) and one fungus (Candida albicans) susceptibility were determined using disc diffusion method. The minimum inhibitory concentration (MIC) of extracts and isolated compounds was determined by broth dilution method. The maximum activity was shown by chloroform extract against C. albicans with a zone of inhibition of 17 mm and minimum activity was displayed by methanolic extract against Pseudomonas sp. with 5 mm. However, isolated compound has shown maximum activity against Pseudomonas sp. with 15 mm. The MIC values higher in methanol extract against Pseudomonas sp. and isolated compound shows good against Pseudomonas sp. and B. subtilis. Our findings indicate that plant could be used as a good antimicrobial agent in food, pharmaceutical and bio-pesticide industries.
机译：该研究旨在检测提取物和分离的达伯利亚脂肪酸化合物的体外抗微生物疗效。将植物叶片（氯仿和甲醇）与柱子的正丁醇分离的植物叶片得到了鉴定为甲氏菌蛋白4'-芦丁苷的黄酮糖苷化合物。不同的提取物和分离的化合物表现出对四种细菌（乙酰丙基丁基鎓，枯草芽孢杆菌，链球菌，链球菌SpseudoMonas Sp的抗细菌和抗真菌多种活动。使用盘扩散法测定一个真菌（Candida albicans）易感性。通过肉汤稀释法测定提取物和分离化合物的最小抑制浓度（MIC）。通过氯仿萃取物针对C.与抑制区域为17mm和最小活性的蛋白质的最大活性显示，甲烷醇提取物对Pseudomonas Sp展开。 5毫米。然而，分离的化合物对假单胞菌SP显示出最大的活性。 15毫米。甲醇提取物较高的麦克风值对Pseudomonas sp。和孤立的化合物展示了对Pseudomonas sp的良好。和B.枯草芽孢杆菌。我们的研究结果表明，植物可以用作食品，制药和生物农药行业的良好抗微生物剂。

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