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首页> 外文会议>Hugh C. Becker Memorial Muskie Symposium >Paternity Analysis of Pooled-Milt Spawning Practices for a Muskellunge Broodstock
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Paternity Analysis of Pooled-Milt Spawning Practices for a Muskellunge Broodstock

机译：Muskellunge Broodstock的汇集米尔特产卵的陪伴分析

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Minnesota Department of Natural Resources biologists use milt from multiple Muskellunge Esox masquinongy males to fertilize eggs of a single female. Protocols recommend multiple males to provide sufficient milt volume and increase genotypic diversity;pooling milt addresses concerns about infertile males and practicality in the lakeside setting where wild adults are spawned. To provide sufficient males, some may be held and reused or their milt may be preserved. Using microsatellite DNA loci, I assessed paternity of fry from 22 lots, each derived from 1 female and 2—6 males. Males had significantly different numbers of offspring in 17 of 22 lots (P < 0.05). Linear mixed models indicated that paternity success of reused males or held milt was only 44% that of new males when in competition, but males also had significantly different success in four of seven lots with all new or reused males. Because this was an assessment of current practices and not experimentally controlled, I could not determinewhat combination of unequal milt volume, unequal sperm count, and sperm competition accounted for the unequal offspring contributions. Regardless of the causes, changes in protocols could be considered to reduce disparities in offspring numbers among males and thus enhance the maintenance of genetic diversity in muskellunge broodstocks.
机译：明尼苏达州自然资源系生物学家使用米尔特来自多个麝香山脉伊斯料理伊斯赛伊斯基金的男性，以施肥卵子的鸡蛋。协议建议多个男性提供足够的摩尔斯体积并增加基因型多样性;汇集米尔在湖洋地区产生狂野成年人的湖边环境中对不育的雄性和实用性的担忧。为了提供足够的男性，有些可能被举行并重复使用，或者他们的摩尔特可能被保留。使用微卫星DNA基因座，我评估了22次肥胖的亲子，每种含量来自1名女性和2-6名男性。男性在22个批次中有17个中的后代数量显着不同（P <0.05）。线性混合模型表明，在竞争中，重复使用的男性或持续的米尔人的陪态性成功仅为新男性的44％，但在七种七种血统中，男性也有明显不同的成功。因为这是对现有实践而非实验控制的评估，我无法确定不平等MILT的数量，不平等的精子计数和精子竞争的组合，占不平等的后代捐款。无论原因如何，协议的变化都可以考虑减少男性中后代数量的差异，从而增强了Muskellunge Brodstocks的遗传多样性的维持。

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