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首页> 外文会议>International Conference on Medical and Biological Engineering >Genetic Polymorphism p-Lactoglobulin Gene in Dubska Pramenka Sheep Breed
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Genetic Polymorphism p-Lactoglobulin Gene in Dubska Pramenka Sheep Breed

机译：Dubska Pramenka羊群中的遗传多态性P-乳酰叶蛋白基因

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Polymorphism exists in P-Iactoglobulin (LGB) milk encoding gene in dairy animals, resulting in presence of different protein isoforms in milk. There is a link between genetic polymorphism in dairy proteins with technological properties of milk, milk yield and allergic reactions in humans. This study was conducted to identify LBG protein isoforms and their frequencies in population of Dubska Pramenka sheep breed. A total of 90 blood samples were genotyped using PCR-RFLP analysis. Genotypes were determined by PCR amplification followed by digestion with Rsal for identification of A and B alleles and using Mspl for C allele identification. Two genetic valiants (A and B) and three genotypes (AA, AB and BB) were identified. The presence of allele C in this study was not noticed. Gene frequencies of LGB A were 13; LGB B were 61; LGB AB were 61. There is no data on the genetic influence on the quality and quantity of milk from Dubska pramenka sheep, and there is need for further studies.
机译：在乳制品中的P-Iacogloblobulin（LGB）牛奶中存在多态性，在乳制品中编码基因，在牛奶中产生不同的蛋白质同种型。乳制品蛋白质遗传多态性与人类的技术特性，人类的技术性质与人类的过敏反应之间存在联系。进行该研究以鉴定Dubska Pramenka羊群中LBG蛋白质同种型及其频率。使用PCR-RFLP分析总共90种血样进行基因分型。通过PCR扩增测定基因型，然后用RSAL消化以鉴定A和B等位基因并使用MSPL进行C等位基因鉴定。鉴定了两个遗传逆（A和B）和三种基因型（AA，AB和BB）。没有注意到这项研究中的等位基因C的存在。 LGB A的基因频率为13; LGB B为61; LGB AB是61.没有关于遗传对Dubska Pramenka羊的质量和数量的遗传影响的数据，并且需要进一步研究。

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