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首页> 外文会议>International Conference in One Health >Expression of Human Neutrophil Gelatinase-Associated Lipocalin (Ngal) on Serum and Urine Sample of Pre Dialysis Kidney Failure Patients
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Expression of Human Neutrophil Gelatinase-Associated Lipocalin (Ngal) on Serum and Urine Sample of Pre Dialysis Kidney Failure Patients

机译：血清血清血清和尿液样品的人性化粒细胞凝胶酶相关脂素（NGAL）的表达

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摘要

Biomarkers for the early prediction of human acute kidney injury (AKI) are clinically important. Recently, neutrophil gelatinase-associated lipocalin (NGAL) has found to be a sensitive biomarker for the prediction of human AKI at a very early stage and the development of AKI after surgery. NGAL is an accurate biosensor to determine kidney function, previous studies have mentioned that NGAL biomarker has been detected in patients with renal failure post cardiac surgery, kidney transplants, and septicemia shock. Therefore the objective of this study is to evaluate the expression of NGAL in serum and urine sample of patients with predialysis renal failure. We collected 10 samples of patients predialysis in Saiful Anwar Hospital which were indicated kidney failure. Then NGAL protein expression was evaluated by SDS PAGE and verified by western blot method. Urea and creatinine serum were analyzed by spectrophotometer method. The data obtained were analyzed descriptively. The results showed that in 10 patients with renal failure predialysis have shown an increase of serum BUN and creatinine above normal. Serum and urine samples of patients were examined by polyacrylamide gel electrophoresis method by a thick protein bands at 25 kDa. While the western blot of NGAL showed positive results expressed by NGAL protein in the samples. In conclusion, the NGAL protein was detected in predialysis patients.
机译：用于早期预测人急性肾损伤（AKI）的生物标志物在临床上是重要的。最近，中性粒细胞明胶酶相关的脂素（NGAL）已发现是一种敏感的生物标志物，用于在术后早期预测人类AKI和手术后AKI的发育。 NGAL是一种准确的生物传感器，用于确定肾功能，先前的研究提到已检测NGAL生物标志物已检测到肾功能衰竭后心脏手术，肾移植和败血症休克的患者。因此，本研究的目的是评估NGAL在血清中的表达和尿液尿液衰竭患者的尿液样本。我们在Saifal Anwar医院收集了10名患者患者样品，该医院显示出肾功能衰竭。然后通过SDS PAGE评估NGAL蛋白表达并通过Western印迹法验证。通过分光光度计法分析尿素和肌酐血清。描述地分析所获得的数据。结果表明，在10例肾功能衰竭患者中，预先血清患者的血清小圆面包和肌酐的增加。通过在25kDa的厚蛋白条带，通过聚丙烯酰胺凝胶电泳方法检查血清和尿液样本。虽然NGAL的Western印迹显示出在样品中的Ngal蛋白表达的阳性结果。总之，在预先检测的患者中检测到NGAL蛋白。

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