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首页> 外文会议>International Conference on Advanced Materials for Better Future >Antibacterial activity study of Attacus atlas cocoon against Staphylococcus aureus and Escherichia coli with diffusion and dilution method
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Antibacterial activity study of Attacus atlas cocoon against Staphylococcus aureus and Escherichia coli with diffusion and dilution method

机译：抗菌阿特拉斯茧对葡萄球菌和大肠杆菌的扩散和稀释法的抗菌活性研究

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The aim of this study was to evaluate the antibacterial activity from Attacus atlas cocoon extract against Gram-negative bacteria (Escherichia coli) and Gram-positive bacteria (Staphylococcus aureus) with 3 diffferent solvents polar, semi-polar and non polar which was ethanol, ethyl acetate and chloroform, also to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extract. Cocoon was extracted with maceration method using 3 solvents with ratio of sample and solvent 1:10. Antibacterial activity of the Extracts obtained was evaluated with Agar disk diffusion method. The best result was then continued to determine the MIC and MBC of the extract using broth macro-dilution method.The results show that each of the extracts have antibacterial activity with broad spectrum against two different type of bacteria at concentration of 1 g/mL with different clear zone between these extracts. Clear zone from the biggest to the smallest against Escherichia coli was ethyl acetate (10.5 mm), chloroform (9 mm) and ethanol (8 mm). While against Staphylococcus aureus, was obtained by chloroform (12.5 mm), ethyl acetate (10.5 mm) and ethanol (7 mm). The MIC value of extracts can not be determine. The smallest MBC value against both bacteria was obtained by ethyl acetate with concentration of 3.125％ b/v as a bactericidal.
机译：本研究的目的是评估从患有革兰阴性细菌（大肠杆菌）和革兰氏阳性细菌（金黄色葡萄球菌）的抗细菌活性，用3个不同的溶剂，乙醇，半极性和非极性，乙酸乙酯和氯仿，还确定提取物的最小抑制浓度（MIC）和最小杀菌浓度（MBC）。使用具有样品和溶剂比例的3个溶剂用浸渍方法提取茧，1:10。用琼脂盘扩散法评价所得提取物的抗菌活性。然后继续使用肉汤宏稀释方法确定最佳结果以确定提取物的MIC和MBC。结果表明，每个提取物具有抗菌活性，其抗菌活性与浓度为1g / ml的两种不同类型的细菌。这些提取物之间的不同清晰区域。从最大的抗大肠杆菌的最小区域含有清晰的区域是乙酸乙酯（10.5mm），氯仿（9mM）和乙醇（8mm）。在对葡萄球菌的同时通过氯仿（12.5mm），乙酸乙酯（10.5mm）和乙醇（7mm）获得。提取物的麦克风值无法确定。对两种细菌的​​最小MBC值是通过乙酸乙酯的浓度为3.125％B / V作为杀菌的乙酸乙酯。

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