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首页> 外文会议>Basic Science International Conference >The Use of Single and Mixed Cultures of Bacillus cereus InaCC B284 and Pseudomonas aeruginosa InaCC B290 to Degrade Used-Cooking Oil
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The Use of Single and Mixed Cultures of Bacillus cereus InaCC B284 and Pseudomonas aeruginosa InaCC B290 to Degrade Used-Cooking Oil

机译：使用单一和混合培养物的芽孢杆菌和混合培养物为484和假单胞菌铜绿假单胞菌均不应复B290降解烹饪油

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Used-cooking oil is a waste product after using cooking oil 3-4 times. The disposal of used cooking oil directly into waterways causes environmental pollution. Bacillus cereus and Pseudomonas aeruginosa are well known to be effective in degrading used-cooking oil. Biodegradation of used-cooking oil is needed to reduce the pollution created by the current disposal of oil. This study aimed to determine the capability of single and mixed cultures of B. cereus InaCC B284 and P. aeruginosa InaCC B290 to degrade used cooking oil. The biodegradation process was carried out using Bushnell Haas Broth (BHB) containing 25% (v/v) used cooking oil for 23 days at room temperature (27-30~°C). Optical density (OD), pH, dissolved oxygen (DO), and fatty acid content were measured throughout the biodegradation process. Different bacterial treatments resulted in significant increases to the OD (p < 0.05) and significant decreases to the pH (p < 0.05) during biodegradation. Qualitative and quantitative fatty acid analysis performed by gas chromatography revealed that a single culture of B. cereus InaCC B284 was capable of degrading seven types of fatty acids, a single culture of P. aeruginosa InaCC B290 was capable of degrading four types of fatty acids, and mixed cultures were capable of degrading 11 types of fatty acids.
机译：使用烹饪油3-4次后，用过的食用油是废物。将二手食用油直接进入水道导致环境污染。均已知芽孢杆菌和假单胞菌铜绿假单胞菌在降解使用的烹饪油中是有效的。需要使用烹饪油的生物降解，以减少通过石油现有处理产生的污染。本研究旨在确定B.Cereus INACC B284和P. Aeruginosa INACC B290的单一和混合培养物的能力降解了食用油。使用23天在室温（27-30〜°C）下使用25％（v / v）23天使用25％（v / v）的Bushnell Haas肉汤（BHB）进行生物降解方法。在整个生物降解过程中测量光密度（OD），pH，溶解氧（DO）和脂肪酸含量。不同的细菌处理导致OD（P <0.05）显着增加，并且生物降解期间对pH（P <0.05）显着降低（P <0.05）。通过气相色谱进行的定性和定量脂肪酸分析表明，B.Cereus INACC B284的单一培养物能够降解七种类型的脂肪酸，单一培养物的P.铜绿假单胞菌INCACC B290能够降解四种类型的脂肪酸，和混合培养物能够降解11种脂肪酸。

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