Detection of Streptococcus uberis Using Dried Milk Samples and qPCR: Comparative with Bacterial Culture in Liquid Milk Samples

机译：使用干牛奶样品和QPCR检测链球菌Uberis：比较液体乳样品中的细菌培养物

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Streptococcus uberis (S. uberis) is an environmental microorganism and one of the main etiological agents associated to bovine mastitis and common intramammary infections in countries with a high milk productivity. A bacteriological culture (BC) of liquid milk sample is considered as the current gold standard methodology for laboratory diagnosis. However, the development of molecular technologies like Real Time PCR (qPCR) and the use of some sample preservation tools such as FTA~Rcards (Whatman [GE Healthcare], NJ, USA)1, offers interesting alternative methods for diagnostics. The aim of this study was to compare two different diagnostic methodologies for diagnostic of S. uberis in milk samples.

机译：链球菌Uberis（uberis）是一种环境微生物和与牛奶生产率高的国家常见的患者患者患有牛乳腺炎和常见的内部感染之一。液体乳样品的细菌培养（BC）被认为是实验室诊断的当前金标准方法。然而，像实时PCR（QPCR）一样的分子技术的发展以及使用一些样品保存工具，如FTA〜RCards（Whatman [GE Healthcare]，NJ，USA）1，为诊断提供了有趣的替代方法。本研究的目的是比较两种不同的诊断方法，用于牛奶样品中的尿布诊断。

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《Annual Meeting of The National Mastitis Council (U.S.).》|2018年|257 p. :|共2页
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1. Diagnostic accuracy of a standardized scheme for identification of Streptococcus uberis in quarter milk samples: A comparison between conventional bacteriological examination, modified Rambach agar medium culturing, and 16S rRNA gene sequencing [J] . Regina Wald, Martina Baumgartner, Verena Urbantke, Journal of dairy science . 2017,第2期

机译：用于鉴定四分之一牛奶样品中乳房链球菌的标准化方案的诊断准确性：常规细菌学检查，改良的Rambach琼脂培养基培养和16S rRNA基因测序之间的比较
2. Multiplex polymerase chain reaction as a mastitis screening test for Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis in bulk milk samples [J] . Patchara Phuektes, Glenn F Browning, Garry Anderson, Journal of dairy research . 2003,第2期

机译：多重聚合酶链反应作为乳腺炎筛查测试散装牛奶样品中的金黄色葡萄球菌，无乳链球菌，痢疾链球菌和乳房链球菌
3. Latent class analysis of real time qPCR and bacteriological culturing for the diagnosis of Streptococcus agalactiae in cow composite milk samples [J] . Holmoy Ingrid H., Toft Nils, Jorgensen Hannah J., Preventive Veterinary Medicine . 2018,第期

机译：实时QPCR潜在阶级分析及细菌培养对牛复合牛奶样品中链球菌胆碱的诊断
4. Detection of Streptococcus uberis Using Dried Milk Samples and qPCR: Comparative with Bacterial Culture in Liquid Milk Samples [C] . Annual Meeting of The National Mastitis Council (U.S.). . 2018

机译：使用干牛奶样品和QPCR检测链球菌Uberis：对液体乳样品中的细菌培养比较
5. I. Development of a passive sampler and static sampling chamber to measure personal exposure to gaseous PAHs. II. Method development for determination of current-use and persistent pesticides in cow milk, human breast milk, baby formulas, and human serum using gas chromatography tandem mass spectrometry. [D] . Chen, Xianyu. 2012

机译：I.开发无源采样器和静态采样室以测量个人对气态多环芳烃的暴露。二。气相色谱串联质谱法测定牛奶，人母乳，婴儿配方食品和人血清中的当前和持久性农药的方法开发。
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机译：基于PCR的直接检测来自亚临床和临床乳制品牛奶样品的链球菌Uberis
7. Identification and further characterization of Streptococcus uberis and Streptococcus parauberis isolated from bovine milk samples [O] . Khan Izhar ul-haq 2002

机译：从牛乳样品中分离出的乳房链球菌和副链球菌的鉴定和进一步表征
8. Grade 'A' Condensed and Dry Milk Ordinance. 1995 Revision. Grade A Condensed andDry Milk Products and Condensed and Dry Whey. Supplement 1 to the Grade A Pasteurized Milk Ordinance, 1995 Recommendations [R] . 1996

机译：“a”级冷凝和干奶条例。 1995年修订。 a级浓缩和干燥乳制品以及浓缩和干燥乳清。 1995年建议书“甲级巴氏杀菌牛奶条例”补编第1号

Detection of Streptococcus uberis Using Dried Milk Samples and qPCR: Comparative with Bacterial Culture in Liquid Milk Samples

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