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首页> 外文会议>International Conference on Materials Engineering and Science >Differentiation of CD34+ Human Hair Follicle Stem Cells into Functional Melanocytes
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Differentiation of CD34+ Human Hair Follicle Stem Cells into Functional Melanocytes

机译：CD34 +人毛卵泡干细胞的分化成功能性黑素细胞

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Skin degenerative diseases, which lower the quality of life has become a colossal challenge across the globe. Despite significant number of such diseases including vitiligo and psoriasis, there has been no great progress in finding a cure to these diseases. Mesenchymal Stem Cells (MSCs) seem promising in this front as MSCs possess unique immunomodulatory properties. There have been preclinical and clinical studies suggesting the use of MSCs for the treatment of atopic dermatitis (AD), a prominent inflammatory skin disease. MSCs can be derived from multiple sources including adipose tissue, dental pulp, umbilical cord, placenta, peripheral blood, endometrium, synovium, and skin. However, stem cells isolated from hair follicle seem to be the closest to the original melanin producing cells. The differentiation of these stromal cells into melanin producing cells holds a promising cellular therapy for skin degenerative diseases. This study aims to differentiate human CD34+ melanocyte stem cells into melanin producing cells. The differentiation has been characterized by probing for the expression of proliferation markers dopachrome tautomerase (DCT ) and microphthalmia associated transcription factor (MITF) and differentiation markers melanocytic antigen (PNL2) and tyrosinase (TYR) through the differentiation process. The results confirm the differentiation of the enriched CD34+ melanocytes into melanin producing cells. Melanin production was confirmed by ELISA to estimate melanin content.
机译：皮肤退行性疾病降低了生活质量已成为全球庞大的挑战。尽管存在大量的这种疾病，包括白癜风和牛皮癣，但在寻找对这些疾病的治疗中没有任何巨大的进展。间充质干细胞（MSCs）似乎在这个前面有望，因为MSCs具有独特的免疫调节性质。临床和临床研究表明MSCs用于治疗特应性皮炎（AD），突出的炎症皮肤病。 MSC可以源自多种来源，包括脂肪组织，牙髓，脐带，胎盘，外周血，子宫内膜，滑膜和皮肤。然而，从毛囊中分离的干细胞似乎是最接近原始黑色素的产生细胞。将这些基质细胞分化为黑色素生成细胞对皮肤退行性疾病具有有前途的细胞疗法。本研究旨在将人CD34 +黑色素细胞干细胞区分为黑色素产生细胞。通过探测通过分化过程探讨增殖标志物Dopachrome互变异物酶（DCT）和微蛋白酶细胞抗原（PNL2）和酪氨酸酶（Tyr）的增殖标志物的表达和微蛋白酶细胞抗原（MITF）和分化标志物（PNL2）和酪氨酸酶（Tyr）的表达。结果证实，将富集的CD34 +黑色素细胞分化为黑色素产生细胞。由ELISA确认黑色素生成以估计黑色素含量。

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