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首页> 外文会议>AOS Australian Conference on Optical Fibre Technology >Submicron Simultaneous IR and Raman Microscopy (IR+Raman): Breakthrough Developments in Optical Photothermal IR (O-PTIR) combined with Raman Provide New Capabilities
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Submicron Simultaneous IR and Raman Microscopy (IR+Raman): Breakthrough Developments in Optical Photothermal IR (O-PTIR) combined with Raman Provide New Capabilities

机译：亚微米同时IR和拉曼显微镜（IR +拉曼）：光学热量IR（O-PTIR）的突破性发展与拉曼相结合，提供了新的能力

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We introduce a new approach to infrared microspectroscopy – Optical-Photothermal Infrared (O-PTIR) Spectroscopy.Based on the principles of the photothermal effect (in response to IR radiation), we describe and illustrate how the noveluse of an optical probe (532nm laser) enables significant enhancements in IR micro-spectroscopy, with ~20ximprovement in spatial resolution compared to traditional IR microscopes (FTIR or direct QCL). Furthermore, thisconfiguration enables the generally preferred and practical reflection (far-field) mode analysis without exhibiting anyscatter artefacts and poor SNR of traditional systems, with O-PTIR reflection spectra being equivalent to FTIRtransmission spectra. Due to the unique systems architecture, namely, the use of a visible probe beam to extract IRspectroscopic information, we show how this same visible laser can be used simultaneously as a Raman excitationsource, thus delivering IR and Raman microspectroscopy from the same spot at the same time with the same spatialresolution. As a performance proof, IR spatial resolution of 400nm across a polymer interface has been obtained,together with 900nm single polymeric particle detection from a microplastics model system from a dried saline solution.Reflection mode fiber analysis of 10 micron and 800nm fibers shows reflection spectra are unaffected by fiber diameterto produce high quality FTIR-transmission mode comparable data. Finally, we demonstrate live cell (epithelial cheekcells) IR spectroscopic imaging analysis in water with submicron intracellular resolution.
机译：我们介绍了一种新的红外线光谱 - 光学光热红外（O-PTIR）光谱的方法。基于光热效应的原理（响应IR辐射），我们描述并说明了新颖的方式使用光学探针（532NM激光）可以实现IR微谱的显着增强，〜20x与传统的IR显微镜（FTIR或Direct QCL）相比，空间分辨率的改善。此外，这一点配置使得通常优选的实际反射（远场）模式分析而不显示任何传统系统的散射艺术品和差的SNR，具有o-ptir反射光谱等同于FTIR传输光谱。由于唯一的系统架构，即使用可见探头光束以提取IR光谱信息，我们展示了如何同时使用相同的可见激光作为拉曼励磁源，从而同时从同一位置从同一位置传递IR和拉曼微型光谱检查解析度。作为一种性能证明，已经获得了在聚合物界面上的400nm的IR空间分辨率，与来自干燥盐水溶液的微塑料模型系统一起与900nm的单一聚合物颗粒检测。10微米和800nm纤维的反射模式纤维分析显示反射光谱不受纤维直径的影响生产高质量的FTIR传输模式可比数据。最后，我们展示了活细胞（上皮脸颊细胞）用亚微米细胞内分辨率的水中的IR光谱成像分析。

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