• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>International Conference on Label-free Biomedical Imaging and Sensing >Prostate Cancer Epithelial Mesenchymal Transition by TGF-β1 exposure monitored with a Photonic Crystal Biosensor
【24h】

Prostate Cancer Epithelial Mesenchymal Transition by TGF-β1 exposure monitored with a Photonic Crystal Biosensor

机译：用光子晶体生物传感器监测TGF-β1暴露的前列腺癌上皮间充质转换

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

During prostate cancer progression, cancerous epithelial cells can undergo epithelial-mesenchymal transition (EMT).EMT is a crucial mechanism for the invasion and metastasis of epithelial tumors characterized by the loss of cell-celladhesion and increased cell mobility. It is associated with biochemical changes such as epithelial cell markers Ecadherinand occludins being down-regulated, and mesenchymal markers vimentin and N-cadherin being upregulated.These changes in protein expression, specifically in the cell membrane, may be monitored via biophysicalprinciples, such as changes in the refractive index (RI) of the cell membrane. In our previous research, wedemonstrated the feasibility of using cellular RI as a unique contrast parameter to accomplish label-free detection ofprostate cancer cells. In this paper, we report the use of our Photonic-Crystal biosensor in a Total-Internal-Reflection(PC-TIR) configuration to construct a label-free biosensing system, which allows for ultra-sensitive quantification ofthe changes in cellular RI due to EMT. We induced prostate cancer cells to undergo EMT by exposing these cells tosoluble Transforming Growth Factor Beta 1 (TGF-β1). The biophysical characteristics of the cellular RI werequantified extensively in comparison to non-induced cancer cells. Our study shows promising clinical potential inutilizing the PC-TIR biosensing system not only to detect prostate cancer cells, but also to evaluate changes in prostatecancer cells due to EMT.
机译：在前列腺癌进展期间，癌性上皮细胞可以进行上皮 - 间充质转换（EMT）。EMT是由细胞 - 细胞丧失的上皮肿瘤的入侵和转移的关键机制粘附性和增加的细胞迁移率。它与生物化学变化如上皮细胞标志物eCadherin相关联和闭塞素下调，间充质标记素和N-cadherin正在上调。可以通过生物物理监测细胞膜的这些蛋白质表达的这些变化原理，例如细胞膜折射率（RI）的变化。在我们以前的研究中，我们展示了使用蜂窝RI作为唯一对比度参数来实现无标签检测的可行性前列腺癌细胞。在本文中，我们报告了在全内反射中使用光子晶体生物传感器（PC-TIR）配置构建无标签的生物传感系统，允许超敏感量化EMT引起的细胞RI的变化。我们诱导前列腺癌细胞通过将这些细胞暴露于eMT可溶转化生长因子β1（TGF-β1）。细胞Ri的生物物理特征是与非诱导的癌细胞相比，广泛定量。我们的研究表明有前途的临床潜力利用PC-TIR生物传感系统不仅要检测前列腺癌细胞，还要评估前列腺的变化由于EMT引起的癌细胞。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号