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EFFECTS OF LOAD ON NORMAL HUMAN OSTEOBLAST FUNCTION

机译:负荷对正常人体骨骨细胞功能的影响

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The effects of load on the secretion and expression of bone markers were tested at different stages of differentiation of primary human osteoblasts. NHOs were both seeded with and without cytodex 3 beads (Sigma),transferred to a NASA rotating wall vessel (modeled microgravity) and harvested at day 7 and day 14. Differentiated and undifferentiated NHOs were loaded at 6-50G for 30 min and compared to cells incubated at 1G after 1 day and 3 days. Collectively the results demonstrate that load has a differential effect on osteoblast differentiation as seen in modeled microgravity and shows specificity in expression of bone cell markers vs. expression of secreted paracrine signaling markers.
机译:在原发性人成骨细胞分化的不同阶段测试载荷对骨标记分泌和表达的影响。 NHOS均接种,并且没有细胞藤虫3珠子(SIGMA),转移到NASA旋转壁容器(建模的微疱疹)并在第7天和第14天收获。分化和未分化的NHO在6-50g上装载30分钟并与1天和3天后在1g孵育细胞。总体上,结果表明,如模拟的微匍匐度所示,载荷对成骨细胞分化具有差异影响,并且显示出骨细胞标记物的表达与分泌的旁静脉信号标记的表达的特异性。

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