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Periplasmic Expression of Single Chain Variable Fragment Against Aflatoxin B1 in Recombinant Escherichia coli

机译：单链可变片段对复合大肠杆菌的黄曲霉毒素B1的周质表达

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Since aflatoxin Bl (AFB1) is a major carcinogen produced in fungi Aspergillus flavus which is frequently found in food and agroproducts, the development of recombinant antibodies specific to AFB1 have been developed for rapid and sensitive detection of AFB1. For recombinant production using E. coli, the variable region genes of heavy (VH) and light (VL) chain fragments were cloned from the murine hybridoma cell line 2C12, and then assembled using a flexible linker, resulting in a single chain variable fragment against AFB1 consisting of VH, (Gly4Ser)3 linker and VL in a row (anti-AFB1 scFv).

机译：由于黄曲霉毒素BL（AFB1）是在食品和农业寄生中经常发现的真菌曲霉属植物意大利菌的主要致癌物质，已经开发了对AFB1的重组抗体的发展，以便快速敏感地检测AFB1。对于使用大肠杆菌的重组产生，从鼠杂交瘤细胞系2C12克隆重（VH）和光（VL）链片段的可变区基因，然后使用柔性接头组装，导致单链可变片段对抗AFB1由VH，（GLY4SER）3连接器和VL连续（抗AFB1 SCFV）组成。

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《PEGS》|2015年||共1页
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Sung-Gun Kim; Yong-Taek Rho; Yong-Deuk Park; Wonki Min; Jin-Ho Seo; Daesang Lee; Woo-Jong Lee;
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中图分类生物工程学（生物技术）;
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1. Procaryotic Expression of Single-Chain Variable-Fragment (scFv) Antibodies: Secretion in L-Form Cells of Proteus mirabilis Leads to Active Product and Overcomes the Limitations of Periplasmic Expression in Escherichia coli [J] . J?rg F. Rippmann, Michaela Klein, Christian Hoischen, Applied and Environmental Microbiology . 1998,第12期

机译：单链可变片段（scFv）抗体的原核表达：奇异变形杆菌L型细胞的分泌导致活性产物，并克服了大肠杆菌中周质表达的局限性
2. Characterisation of monoclonal antibody against aflatoxin B_1 produced in hybridoma 2C12 and its single-chain variable fragment expressed in recombinant Escherichia coli [J] . Won-Ki Min, Dae-Hyuk Kweon, Kyungmoon Park, Food Chemistry . 2011,第3期

机译：杂交瘤细胞2C12中产生的抗黄曲霉毒素B_1单克隆抗体及其在重组大肠杆菌中表达的单链可变片段的表征
3. Construction, expression and characterisation of a single chain variable fragment in the Escherichia coli periplasmic that recognise MCF-7 breast cancer cell line [J] . Elham Omer Mahgoub, Ahmed Kamal Bolad Journal of Cancer Research and Therapeutics . 2014,第2期

机译：识别MCF-7乳腺癌细胞系的大肠杆菌周质中单链可变片段的构建，表达和表征
4. Periplasmic Expression of Single Chain Variable Fragment Against Aflatoxin B1 in Recombinant Escherichia coli [C] . Sung-Gun Kim, Yong-Taek Rho, Yong-Deuk Park, PEGS . 2015

机译：单链可变片段对复合大肠杆菌的黄曲霉毒素B1的周质表达
5. Expression of a recombinant copper/zinc superoxide dismutase (SOD-1): Tetanus toxin C-fragment hybrid protein in Escherichia coli for the targeted delivery of enzyme to neurons. [D] . Francis, Jonathan William. 1995

机译：重组铜/锌超氧化物歧化酶（SOD-1）：破伤风毒素C片段杂合蛋白在大肠杆菌中的表达，用于酶向神经元的靶向递送。
6. Procaryotic Expression of Single-Chain Variable-Fragment (scFv) Antibodies: Secretion in L-Form Cells of Proteus mirabilis Leads to Active Product and Overcomes the Limitations of Periplasmic Expression in Escherichia coli [O] . Jörg F. Rippmann, Michaela Klein, Christian Hoischen, 1998

机译：单链可变片段（scFv）抗体的原核表达：奇异变形杆菌L型细胞的分泌导致活性产物并克服了大肠杆菌中周质表达的局限性
7. Construction, expression and characterisation of a single chain variable fragment in the Escherichia coli periplasmic that recognise MCF-7 breast cancer cell line [O] . Elham Omer Mahgoub, Ahmed Kamal Bolad 2014

机译：识别mCF-7乳腺癌细胞系的大肠杆菌周质中单链可变片段的构建，表达和表征

Periplasmic Expression of Single Chain Variable Fragment Against Aflatoxin B1 in Recombinant Escherichia coli

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