Efficient Expression and Purification of Bombina maxima Trefoil factors 2 Variant in Escherichia coli as fusion protein

机译：大肠杆菌中Bombina Maxima Trefoil因子2变种作为融合蛋白的高效表达及纯化

获取原文

页面导航

摘要
著录项
相似文献
相关主题

摘要

Bm-TFF2 was isolated from Bombina maxima. The B.maxima Trefoil factors 2 Variant (vBm-TFF2) could be developed as a novel administered wound healing agent. The challenge is its preparation from refolding insoluble protein expressed in Escherichia coli. A recombinant vBm-TFF2 was overexpressed in E. coli cells as a fusion protein with bacterial N-utilizing substance A (NusA). The fusion protein NusA-Bm-TFF2 can promote the wound healing.

机译：BM-TFF2从Bombina Maxima隔离。 B.Maxima Trefoil因子2变体（VBM-TFF2）可以作为新型给药伤口愈合剂开发。挑战是它从重新折叠在大肠杆菌中表达的不溶性蛋白质的制备。重组VBM-TFF2在大肠杆菌细胞中过表达，作为融合蛋白，具有细菌N-利用物质A（NUSA）。融合蛋白NUSA-BM-TFF2可以促进伤口愈合。

著录项

来源
《International Conference on Materials Science and Computational Engineering》|2014年||共4页
会议地点
作者
Tongyi Sun;
展开▼
作者单位

展开▼
会议组织
原文格式 PDF
正文语种
中图分类 TB3-53;
关键词
Bm-TFF2; Expression; Purification; Escherichia coli;

机译：BM-TFF2;表达;纯化;埃尔希希亚大肠杆菌;

相似文献

外文文献
中文文献
专利

1. Erratum: High level expression, purification and characterization of active fusion human C1q and tumor necrosis factor related protein 2 (hCTRP2) in Escherichia coli (Protein Expression and Purification (2011) 79 (1-6)) [J] . Li H., Gao X., Zhou Y., Protein Expression and Purification . 2012,第1期

机译：勘误：活性融合人C1q和肿瘤坏死因子相关蛋白2（hCTRP2）在大肠杆菌中的高水平表达，纯化和鉴定（蛋白质表达和纯化（2011）79（1-6））
2. Highly efficient soluble expression and purification of recombinant human basic fibroblast growth factor (hbFGF) by fusion with a new collagen-like protein (Scl2) in Escherichia coli [J] . Quantum electronics . 2020,第6期

机译：通过融合在大肠杆菌中的新胶原样蛋白（SCL2）融合来高效可溶性表达和纯化重组人类碱性成纤维细胞生长因子（HBFGF）
3. High level expression, purification and characterization of active fusion human C1q and tumor necrosis factor related protein 2 (hCTRP2) in Escherichia coli [J] . Li H., Gao X., Zhou Y., Protein Expression and Purification . 2011,第1期

机译：活性融合人C1q和肿瘤坏死因子相关蛋白2（hCTRP2）在大肠杆菌中的高水平表达，纯化和鉴定
4. Efficient Expression and Purification of Bombina maxima Trefoil factors 2 Variant in Escherichia coli as fusion protein [C] . Tongyi Sun International Conference on Materials Science and Computational Engineering . 2014

机译：大肠杆菌中Bombina Maxima Trefoil因子2变种作为融合蛋白的高效表达及纯化
5. The overexpression, purification, and structural characterization of the Escherichia coli integral membrane protein glycerol facilitator [D] . Manley, Darren Mark 2002

机译：大肠杆菌整合膜蛋白甘油促进剂的过表达，纯化和结构表征
6. Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline [O] . Stephen N. Hewitt, Ryan Choi, Angela Kelley, 2011

机译：蛋白质在麦芽糖中的表达与麦芽糖结合蛋白的融合体可在高通量蛋白质表达和纯化流程中挽救未表达的靶标
7. Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline [O] . Hewitt, Stephen N., Choi, Ryan, Kelley, Angela, 2011

机译：蛋白质在麦芽糖中的表达与麦芽糖结合蛋白的融合体，可在高通量蛋白质表达和纯化流程中挽救未表达的靶标

Efficient Expression and Purification of Bombina maxima Trefoil factors 2 Variant in Escherichia coli as fusion protein

摘要

著录项

相似文献

相关主题

期刊订阅