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首页> 外文会议>National Fusarium Head Blight Forum Conference >QTL CONFERRING TYPE II RESISTANCE TO FUSARIUM HEAD BLIGHT IN ADAPTED WHEAT CULTIVAR 'UI STONE' AND ITS EFFECT ON YIELD
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QTL CONFERRING TYPE II RESISTANCE TO FUSARIUM HEAD BLIGHT IN ADAPTED WHEAT CULTIVAR 'UI STONE' AND ITS EFFECT ON YIELD

机译：QTL赋予II型耐镰刀菌头枯萎，适应小麦品种“UI石”及其对产量的影响

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Fusarium head blight (FHB) is an emerging wheat disease in Southeastern Idaho. Most cultivars grown in this region are susceptible to FHB. Several exotic sources of resistance are available to fill this hole, however, lack of adaptation and often complication arises due to "linkage drag" limit their direct utilization. Therefore, identification and development of adapted FHB resistant cultivars is one of major objectives in the University of Idaho wheat breeding program. The objectives of this studywere to map QTL associate with type II FHB resistance in the adapted soft white spring (SWS) wheat cultivar 'UI Stone' and evaluate side effect of identified QTL on yield. A total of 151(F_(46)) recombinant inbred lines (RILs) derived from the cross between resistant cultivar 'UI Stone' and a moderately susceptible cultivar' Alturas' were evaluated for type II FHB resistance by measuring disease severity expressed as a percentage of infected spikelets (PIS) in four greenhouse experiments over three years. The RILs were genotyped with 154 markers (77 SSR and 77 SNP). A Linkage map was constructed using MapMaker 3.0b and QTL analysis was performed using WinQTL Cartographer Ver. 2.5. Two major QTL for type II FHB resistance, QFhbuis.ab-2B and QFhbuis.abSB, were identified by both single marker analysis and composite interval mapping (CIM) methods and the two QTL together explained 23.6 to 24.8% of phenotypic variation. These QTL had no significant effect on yield based on the regression analyses. This study also identified 4 lines with better FHB resistance and higher grain yield than UI Stone. These four lines could be used as germplasm and/or released as new resistant cultivars after further evaluation. More phenotypic evaluation for FHB severity by cooperators in 2015 and 90K SNP marker data will be utilized to validate the effect of these two QTL for potential application in marker-assisted breeding.
机译：赤霉病（FHB）是爱达荷州东南部一个新兴的小麦病害。在这一地区种植的品种大多是容易FHB。电阻的几个异国源可用于填补这个空洞，但是，缺乏适应，往往会出现复杂因“连锁累赘”限制其直接利用。因此，识别和适应赤霉病抗病品种的发展是在爱达荷州大学小麦育种计划的主要目标之一。此studywere的目标，以与类型II赤霉病抗性QTL映射关联在适于软白春小麦（SWS）小麦品种“UI石”和评估鉴定QTL的对产量的副作用。总共有151（F_（46））的重组近交系（RIL）从抗性品种“UI石”和适度感病品种”阿尔图拉斯之间的杂交衍生为式II FHB性的评价通过测定疾病的严重程度，以百分比表示的四个温室试验在三年内感染的小穗（PIS）。所述重组自交系用154个标记（SSR 77和77 SNP）基因分型。使用地图制作3.0b中构建的遗传连锁图，并使用WinQTL制图版进行QTL分析。 2.5。对于类型II FHB阻力两个主效QTL，QFhbuis.ab-2B和QFhbuis.abSB中，通过两个单标记分析和复合区间作图法（CIM）方法鉴定和两个QTL一起解释23.6表型变异的24.8％。这些QTL对产量没有影响显著基于回归分析。本研究还发现4行更好FHB电阻和更高的产量比UI石。这四条线可被用作种质和/或释放为进一步评估后新抗病品种。通过在2015和合作者90K SNP标记数据FHB严重性多个表型评价将被用于验证这两个QTL的用于标记辅助的育种潜在的应用效果。

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