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首页> 外文会议>International Seminar on Smart Molecule of Natural Resources >Production and Optimization of Lipase by Aspergillus niger using Coconut Pulp Waste in Solid State Fermentation
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Production and Optimization of Lipase by Aspergillus niger using Coconut Pulp Waste in Solid State Fermentation

机译：用椰子纸浆废料在固态发酵中生产和优化脂肪酶的生产和优化

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Lipase has high economical value and an attribute that treasures wide range applications. Among the microbes, fungi to be the best source for lipase production in agroindustrial residue using solid state fermentation (SSF). One of agro-industrial residue is coconut pulp waste. The aims of this study is to explore the possibility of coconut pulp waste for producing of lipase using fungi isolate, Aspergillus niger and find the partial optimum condition of lipase production based on 2 parameters only. The crude enzyme was extracted by mixing fermented substrate with 50 mL sterile aquades pH 7 containing 0.01% Tween-80. The protein concentrations are determined by Bradford method using spectrophotometric. Lipase activity was followed the titrimetric method. The SSF was carried out with variation of initial spore concentration of 1×10~5, 1×10~6, 1×10~7, and 1×10~8 (spores.mL~(-1)) and crude enzyme was harvested by adding 25, 50, 75, 100, and 125 (mL) of extract solution. The result showed that initial spore concentration and extract solution affected of lipase production. The SSF in coconut pulp waste using A. niger has successfully produced lipase with optimum condition were achieved by adding spore concentration inoculum 1×10~6 spore.mL~(-1) in acidic condition at 30°C for 7 days and extracted by 75 mL of extract solution that was result 72.5 mL of crude enzyme with lipase activity 10.83 U.mL~(-1) and protein content 0.074 mg/mL~(-1).
机译：脂肪酶具有很高的经济价值和珍品宽范围应用的属性。在微生物中，真菌是使用固态发酵（SSF）的农业工业残留物中脂肪酶生产的最佳来源。农业工业残留物之一是椰子浆料废物。本研究的目的是探讨使用真菌分离物，曲霉菌株的脂肪酶生产糖粉浆料废物的可能性，并仅基于2个参数找到脂肪酶生产的部分最佳条件。通过将发酵底物混合，用50ml无菌Abades含有0.01％Tween-80的PH 7萃取粗酶。通过使用分光光度法的Bradford方法测定蛋白质浓度。脂肪酶活性跟随滴定法。使用初始孢子浓度为1×10〜5，1×10〜6,1×10〜7和1×10〜8（孢子〜（-1））和粗酶的变化进行了SSF通过加入25,50,75,100和125（mL）的提取物溶液收获。结果表明，初始孢子浓度和提取液对脂肪酶生产影响。使用A. niger的椰子纸浆废物中的SSF通过在30℃下在酸性条件下将孢子浓度接种物1×10〜6孢子添加7天并提取而成功地产生了最佳状态的脂肪酶。通过75毫升的提取溶液，其结果为72.5ml酸碱活性10.83 u.ml〜（-1）和蛋白质含量0.074mg / ml〜（-1）。

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