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Raman spectroscopy for the assessment of acute myeloid leukemia: a proof of concept study

机译：拉曼光谱法评估急性髓细胞性白血病：概念研究的证明

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摘要

Acute myeloid leukemia (AML) is a proliferative neoplasm, that if not properly treated can rapidly cause a fatal outcome. The diagnosis of AML is challenging and the first diagnostic step is the count of the percentage of blasts (immature cells) in bone marrow and blood sample, and their morphological characterization. This evaluation is still performed manually with a bright field light microscope. Here we report results of a study applying Raman spectroscopy for analysis of samples from two patients affected by two AML subtypes characterized by a different maturation stage in the neutrophilic lineage. Ten representative cells per sample were selected and analyzed with high-resolution confocal Raman microscopy by scanning 64×64 (4096) points in a confocal layer through the volume of the whole cell. The average spectrum of each cell was then used to obtain a highly reproducible mean fingerprint of the two different AML subtypes. We demonstrate that Raman spectroscopy efficiently distinguishes these different AML subtypes. The molecular interpretation of the substantial differences between the subtypes is related to granulocytic enzymes (e.g. myeloperoxidase and cytochrome b_(558)), in agreement with different stages of maturation of the two considered AML subtypes . These results are promising for the development of a new, objective, automated and label-free Raman based methods for the diagnosis and first assessment of AML.
机译：急性髓细胞性白血病（AML）是一种增殖性肿瘤，如果治疗不当，会迅速导致致命的后果。 AML的诊断具有挑战性，第一步诊断是计算骨髓和血液样本中胚细胞（未成熟​​细胞）的百分比及其形态学特征。仍然使用明场光学显微镜手动执行此评估。在这里，我们报告了一项应用拉曼光谱分析来自两名患者的样本的研究结果，该两名患者受到两种以中性粒细胞不同成熟阶段为特征的AML亚型的影响。每个样品选择十个代表性细胞，并通过扫描共聚焦层中整个细胞体积的64×64（4096）个点，用高分辨率共聚焦拉曼显微镜进行分析。然后，将每个细胞的平均光谱用于获得两种不同AML亚型的高度可再现的平均指纹。我们证明了拉曼光谱能有效地区分这些不同的AML亚型。亚型之间实质性差异的分子解释与颗粒细胞酶（例如髓过氧化物酶和细胞色素b_（558））有关，这与两种被认为是AML亚型成熟的不同阶段相一致。这些结果对于开发一种新的，客观的，自动化的，无标签的基于拉曼的方法进行AML的诊断和首次评估很有希望。

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  • 来源
    《》|2014年|89390F.1-89390F.10|共10页
  • 会议地点 San Francisco CA(US)
  • 作者

    R. Vanna; C. Tresoldi; P. Ronchi; A.T.M. Lenferink; C. Morasso; D. Mehn; M. Bedoni; L. W. M. M. Terstappen; F. Ciceri; C. Otto; F. Gramatica;

  • 作者单位

    Laboratory of Nanomedicine and Clinical Biophotonics, Fond. Don Carlo Gnocchi ONLUS, Piazzale Morandi 6, 20121, Milan, Italy, Medical Cell Bio Physics, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Dienstweg 1, 7522ND, Enschede, The Netherlands;

    Hematology and Bone Marrow Transplantation Unit, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy;

    Hematology and Bone Marrow Transplantation Unit, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy;

    Medical Cell Bio Physics, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Dienstweg 1, 7522ND, Enschede, The Netherlands;

    Laboratory of Nanomedicine and Clinical Biophotonics, Fond. Don Carlo Gnocchi ONLUS, Piazzale Morandi 6, 20121, Milan, Italy;

    Laboratory of Nanomedicine and Clinical Biophotonics, Fond. Don Carlo Gnocchi ONLUS, Piazzale Morandi 6, 20121, Milan, Italy;

    Laboratory of Nanomedicine and Clinical Biophotonics, Fond. Don Carlo Gnocchi ONLUS, Piazzale Morandi 6, 20121, Milan, Italy;

    Medical Cell Bio Physics, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Dienstweg 1, 7522ND, Enschede, The Netherlands;

    Hematology and Bone Marrow Transplantation Unit, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy;

    Medical Cell Bio Physics, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, Dienstweg 1, 7522ND, Enschede, The Netherlands;

    Laboratory of Nanomedicine and Clinical Biophotonics, Fond. Don Carlo Gnocchi ONLUS, Piazzale Morandi 6, 20121, Milan, Italy;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    label-free; blasts; hematopoietic; myeloperoxidase; cancer; tumor; AML; Raman;

    机译：无标签；爆炸造血的髓过氧化物酶癌症;瘤;反洗钱;拉曼;

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