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Microfluidic cell culture systems with integrated sensors for drug screening

机译:带有集成传感器的微流细胞培养系统,用于药物筛选

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Cell-based testing is a key step in drug screening for cancer treatments. A microfluidic platform can permit more precise control of the cell culture microenvironment, such as gradients in soluble factors. These small-scale devices also permit tracking of low cell numbers. As a new screening paradigm, a microscale system for integrated cell culture and drug screening promises to provide a simple, scalable tool to apply standardized protocols used in cellular response assays. With the ability to dynamically control the microenvironment, we can create temporally varying drug profiles to mimic physiologically measured profiles. In addition, low levels of oxygen in cancerous tumors have been linked with drug resistance and decreased likelihood of successful treatment and patient survival. Our work also integrates a thin-film oxygen sensor with a microfluidic oxygen gradient generator which will in future allow us to create spatial oxygen gradients and study effects of hypoxia on cell response to drug treatment. In future, this technology promises to improve cell-based validation in the drug discovery process, decreasing the cost and increasing the speed in screening large numbers of compounds.
机译:基于细胞的测试是癌症治疗药物筛选中的关键步骤。微流体平台可以允许更精确地控制细胞培养微环境,例如可溶性因子的梯度。这些小型设备还允许跟踪低细胞数。作为一种新的筛选范例,用于细胞整合培养和药物筛选的微型系统有望提供一种简单,可扩展的工具,以应用细胞反应测定中使用的标准化方案。具有动态控制微环境的能力,我们可以创建随时间变化的药物概况,以模仿生理测量的概况。另外,癌性肿瘤中的低水平的氧气与耐药性相关联,并降低了成功治疗和患者存活的可能性。我们的工作还将薄膜氧传感器与微流氧梯度发生器集成在一起,将来将使我们能够创建空间氧梯度并研究缺氧对药物治疗细胞反应的影响。将来,这项技术有望在药物开发过程中改善基于细胞的验证,从而降低成本并提高筛选大量化合物的速度。

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