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首页> 外文会议>Multiphoton Microscopy in the Biomedical Sciences VI; Progress in Biomedical Optics and Imaging; vol.7 no.12 >CARS microscopy for the monitoring of fat deposition mechanisms in a living organism
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CARS microscopy for the monitoring of fat deposition mechanisms in a living organism

机译：CARS显微镜用于监测生物体内脂肪沉积的机制

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We introduce near-infrared Coherent Anti-Stokes Raman Scattering (CARS) microscopy as a method for the monitoring of fat deposition in a living organism by directly probing the CH_2 vibration of the lipids without the need for staining or labeling. This study nicely brings forward all the advantages of the technique: deep probe depth, low excitation powers, high 3-dimensional resolution, and visualization without the interference of exogenous label molecules, or fixation and staining procedures. Differences in fat deposition during the life cycle of the nematode Caenorhabditis elegans were evaluated quantitatively from the CARS microscopy images, showing that the technique can be used to study mechanisms that regulate lipid storage. Beside the wild type nematode, the feeding-deficient mutant pha-3 was studied. It was shown that the embryonal accumulation of energy stores is enough for the development of a full-sized pre-adult larva, being possible also for the mutant. However, the volume density of lipid stores at the fourth and last pre-adult development stage seems to determine its adult body size. Whereas the wild type larva maintains its size when becoming adult, though at the cost of reduced lipid density, the feeding deficient mutant instead has to reduce its body size in order to reach the same volume density of lipid stores. Both strains start off their adult life with a volume fraction of lipid stores corresponding to 6-7%; the wild type with a radius of 24±2 μm and the pha-3 mutant with a significantly smaller radius of 16±3 μm.
机译：我们引入近红外相干抗斯托克斯拉曼散射（CARS）显微镜作为一种通过直接探测脂质的CH_2振动而无需染色或标记的方法来监测生物体内脂肪沉积的方法。这项研究很好地提出了该技术的所有优点：深探针深度，低激发功率，高3维分辨率和可视化效果而不受外源标记分子或固定和染色程序的干扰。线虫秀丽隐杆线虫的生命周期中脂肪沉积的差异已从CARS显微镜图像中定量评估，表明该技术可用于研究调节脂质储存的机制。除野生型线虫外，还研究了饲喂缺陷型突变体pha-3。结果表明，能量储存的胚胎积累足以形成完整的成年幼虫，对于突变体也是可能的。然而，脂质在成人的第四个发育阶段和最后一个发育阶段的体积密度似乎决定了其成人的体型。尽管成年后野生型幼虫保持其大小，尽管以降低脂质密度为代价，但缺乏饲料的突变体却不得不减小其体型，以达到相同的脂质贮存体密度。两种菌株的成年期都以脂质存储量的6-7％为基础；半径为24±2μm的野生型和pha-3突变体，半径为16±3μm明显较小。

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