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首页> 外文会议>Multiphoton Microscopy in the Biomedical Sciences VII; Progress in Biomedical Optics and Imaging; vol.8 no.19; Proceedings of SPIE-The International Society for Optical Engineering; vol.6442 >CARS imaging with a new, 532 nm synchronously pumped picosecond OPO
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CARS imaging with a new, 532 nm synchronously pumped picosecond OPO

机译：使用新的532 nm同步泵浦皮秒OPO进行CARS成像

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摘要

A new, synchronously pumped picosecond OPO for CARS microscopy is presented. It is based on non-critically phasematched interaction in LBO pumped by a frequency-doubled modelocked Nd:Vanadat laser at 532 nm. Within the parametric process a tuneable pair of two different wavelengths in the NIR range is generated (Signal ＜ 680 … 990 nm, Idler 1150 … ＞ 2450 nm). In this system they are extracted from the cavity at the same mirror and therefore propagating collinear at the same beam path. Due to the mechanism of their generation there is no jitter between Signal and Idler. Though the wavelengths are different the GVD is negligible for this picosecond pulse duration. As a result the two pulse trains are spatially and temporally perfectly matched. The pulses generated are close to transform limit with about 5-6 ps pulse duration, excellent beam quality (M~2 ＜ 1,1) and high pointing stability. The output power for Signal and Idler is about 1 W each @ 4 W pump power. The tuning mechanism is split into two parts - temperature tuning for rough variations and fast angular BRF tuning for the fine adjustment of the output wavelength. The perfect spatial and temporal overlap make the described OPO an ideal and nearly hands-free laser source for CARS microscopy with a tuneable energy difference 1,400 … ＞ 10,000 cm~(-1). The absolute wavelength range is resulting in high penetration depth and low photo damage of the analyzed samples. Finally some CARS-images are presented and the latest results and methods for further sensitivity enhancements are shown.
机译：提出了一种用于CARS显微镜的新型同步泵浦皮秒OPO。它基于倍频锁模的Nd：Vanadat激光器在532 nm泵浦的LBO中的非临界相位匹配相互作用。在参量过程中，会生成可调谐的NIR范围内的两个不同波长对（信号＜680…990 nm，惰轮1150…＞ 2450 nm）。在此系统中，它们是从同一反射镜的腔中提取的，因此在同一光束路径下共线传播。由于它们的生成机制，Signal和Idler之间没有抖动。尽管波长不同，但是对于该皮秒脉冲持续时间，GVD可以忽略不计。结果，两个脉冲串在空间和时间上完美匹配。产生的脉冲接近转换极限，脉冲持续时间约为5-6 ps，光束质量极佳（M〜2 ＜1,1），指向稳定性高。泵浦功率为4 W时，信号和惰轮的输出功率分别约为1W。调整机制分为两部分-温度调整（用于粗略变化）和快速角度BRF调整（用于微调输出波长）。完美的空间和时间重叠使所描述的OPO成为CARS显微镜的理想且几乎免提的激光源，其能量差可调节为1,400…＞ 10,000 cm〜（-1）。绝对波长范围导致被分析样品的高穿透深度和低光损伤。最后介绍了一些CARS图像，并显示了进一步提高灵敏度的最新结果和方法。

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