A Femtosecond Stimulated Raman Loss (fSRL) Microscope for Highly Sensitive Bond-Selective Imaging

机译：飞秒受激拉曼损耗（fSRL）显微镜用于高灵敏度键选择成像

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We demonstrate nonlinear vibrational imaging of isolated Raman bands by detecting femtosecond pulse stimulated Raman loss. Femtosecond pulse excitation produces a stimulated Raman loss signal that is 12 times larger than what picosecond pulse excitation produces. The strong signal allowed real-time, bond-selective imaging of deuterated palmitic acid-d_(31) inside live cells, and 3D sectioning of fat storage in live C. elegans. With the high peak power provided by femtosecond pulses, this system is highly compatible with other nonlinear optical modalities such as two-photon excited fluorescence. With most of the excitation power contributed by the Stokes beam in the 1.0 - 1.2 urn wavelength range, photodamage of biological samples was not observed.

机译：我们通过检测飞秒脉冲激发的拉曼损耗来演示孤立拉曼带的非线性振动成像。飞秒脉冲激发产生的受激拉曼损耗信号比皮秒脉冲激发产生的大12倍。强大的信号可以对活细胞内的氘代棕榈酸-d_（31）进行实时，键选择性成像，并可以对秀丽隐杆线虫中的脂肪进行3D切片。飞秒脉冲提供的高峰值功率使该系统与其他非线性光学模态（如双光子激发荧光）高度兼容。由于斯托克斯光束在1.0-1.2微米波长范围内贡献的大部分激发功率，未观察到生物样品的光损伤。

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《Multiphoton microscopy in the biomedical sciences XI》|2011年|p.79032L.1-79032L.8|共8页
会议地点 San Francisco CA(US)
作者
Delong Zhang; Mikhail N. Slipchenko; Shuhua Yue; Junjie Li; Ji-Xin Cheng;
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作者单位

Department of Chemistry West Lafayette, IN 47907;

Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907;

Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907;

Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907;

Department of Chemistry West Lafayette, IN 47907,Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907;

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原文格式 PDF
正文语种 eng
中图分类医用物理学;
关键词
stimulated raman scattering (srs); nonlinear microscopy; multimodal imaging; femtosecond laser; coherent raman scattering; lipid;

机译：拉曼散射（srs）；非线性显微镜多模态成像飞秒激光相干拉曼散射脂类;

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专利

1. Label-free imaging of lipid-droplet intracellular motion in early Drosophila embryos using femtosecond-stimulated Raman loss microscopy [J] . DouW., ZhangD., JungY., Biophysical Journal . 2012,第7期

机译：使用飞秒刺激的拉曼损失显微镜对果蝇早期胚胎中脂质滴的细胞内运动进行无标记成像
2. Highly sensitive detection of near-field Raman scattered light from strained Si/SiGe heterostructures by scanning near-field optical Raman microscope using ultraviolet resonant Raman scattering [J] . M. Yoshikawa, M. Murakami, H. Ishida Applied Physicsletters . 2008,第9期

机译：通过使用紫外共振拉曼散射扫描近场光学拉曼显微镜，高灵敏度地检测应变Si / SiGe异质结构中的近场拉曼散射光
3. Sagnac-enhanced impulsive stimulated Raman scattering for highly sensitive low-frequency Raman spectroscopy [J] . Peterson Walker, Hiramatsu Kotaro, Goda Keisuke Optics Letters . 2019,第21期

机译：SAGNAC增强的冲动刺激式拉曼散射，用于高敏感的低频拉曼光谱
4. A Femtosecond Stimulated Raman Loss (fSRL) Microscope for Highly Sensitive Bond-Selective Imaging [C] . Delong Zhang, Mikhail N. Slipchenko, Shuhua Yue, Conference on multiphoton microscopy in the biomedical sciences . 2011

机译：用于高灵敏度结合选择性成像的飞秒刺激拉曼损失（FSRL）显微镜
5. Design, operation and applications of a visible-light confocal scanning Fourier transform Raman microscope for volumetric Raman spectrochemical imaging. [D] . Brenan, Colin John Herbert. 1997

机译：用于体积拉曼光谱化学成像的可见光共聚焦扫描傅里叶变换拉曼显微镜的设计，操作和应用。
6. Highly Sensitive Vibrational Imaging by Femtosecond Pulse Stimulated Raman Loss [O] . Delong Zhang, Mikhail N. Slipchenko, Ji-Xin Cheng -1

机译：由飞秒脉冲刺激拉曼损失的高度敏感的振动成像
7. Highly Sensitive Vibrational Imaging by Femtosecond Pulse Stimulated Raman Loss [O] . Delong Zhang, Mikhail N. Slipchenko, Ji-Xin Cheng 2011

机译：由飞秒脉冲刺激拉曼损失的高度敏感的振动成像

A Femtosecond Stimulated Raman Loss (fSRL) Microscope for Highly Sensitive Bond-Selective Imaging

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