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首页> 外文会议>Conference on multiphoton microscopy in the biomedical sciences >A Femtosecond Stimulated Raman Loss (fSRL) Microscope for Highly Sensitive Bond-Selective Imaging
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A Femtosecond Stimulated Raman Loss (fSRL) Microscope for Highly Sensitive Bond-Selective Imaging

机译：用于高灵敏度结合选择性成像的飞秒刺激拉曼损失（FSRL）显微镜

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摘要

We demonstrate nonlinear vibrational imaging of isolated Raman bands by detecting femtosecond pulse stimulated Raman loss. Femtosecond pulse excitation produces a stimulated Raman loss signal that is 12 times larger than what picosecond pulse excitation produces. The strong signal allowed real-time, bond-selective imaging of deuterated palmitic acid-d_(31) inside live cells, and 3D sectioning of fat storage in live C. elegans. With the high peak power provided by femtosecond pulses, this system is highly compatible with other nonlinear optical modalities such as two-photon excited fluorescence. With most of the excitation power contributed by the Stokes beam in the 1.0 - 1.2 urn wavelength range, photodamage of biological samples was not observed.
机译：通过检测飞秒脉冲刺激拉曼损失，我们展示了隔离拉曼带的非线性振动成像。飞秒脉冲激励产生刺激的拉曼损耗信号，其比Pic秒脉冲激励产生的12倍。强信号允许实时，活细胞内氘代棕榈酸-D_（31）的粘合选择性成像，以及在Live C.杆杆线上的脂肪储存的3D切片。利用由飞秒脉冲提供的高峰功率，该系统与其他非线性光学模式高度相容，例如双光子激发荧光。由于大多数由斯托克斯梁在1.0-1.2瓮波长范围内贡献的大部分激励力，未观察到生物样品的光局部。

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