• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Multiphoton microscopy in the biomedical sciences XI >Multiwavelength FLIM: New applications and algorithms
【24h】

Multiwavelength FLIM: New applications and algorithms

机译：多波长FLIM：新的应用程序和算法

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The combination of time-resolved and spectral resolved techniques as achieved by SLIM (spectrally resolved fluorescence lifetime imaging) improves the analysis of complex situations, when different fluorophores have to be distinguished. This could be the case when endogenous fluorophores of living cells and tissues are observed to identify the redox state and oxidative metabolic changes of the mitochondria. Other examples are FRET (resonant energy transfer) measurements, when different donor/acceptor pairs are observed simultaneously. SLIM is working in the time domain employing excitation with short light pulses and detection of the fluorescence intensity decay in many cases with time-correlated single photon counting (TCSPC). Spectral resolved detection is achieved by a polychromator in the detection path and a 16-channel multianode photomultiplier tube with the appropriate routing electronics. Within this paper special attention will be focused on FRET measurements with respect to protein interactions in Alzheimers disease. Using global analysis as the phasor plot approach or integration of the kinetic equations taking into account the multidimensional datasets in every spectral channel we could demonstrate considerable improvement of our calculations.
机译：当必须区分不同的荧光团时，通过SLIM（光谱分辨荧光寿命成像）实现的时间分辨技术和光谱分辨技术的组合可改善对复杂情况的分析。当观察到活细胞和组织的内源性荧光团以识别线粒体的氧化还原状态和氧化代谢变化时，可能就是这种情况。其他示例是当同时观察到不同的供体/受体对时的FRET（共振能量转移）测量。 SLIM在时域中使用短光脉冲激发并在许多情况下通过与时间相关的单光子计数（TCSPC）检测荧光强度衰减来进行工作。光谱分辨的检测是通过检测路径中的多色仪和带有适当路由电子设备的16通道多阳极光电倍增管实现的。在本文中，将特别关注与阿尔茨海默氏病中蛋白质相互作用有关的FRET测量。使用全局分析作为相量图方法或动力学方程式的积分，并考虑到每个光谱通道中的多维数据集，我们可以证明我们的计算有很大的改进。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号