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Optimal repetition rate and pulse duration studies for two photon imaging

机译:两个光子成像的最佳重复率和脉冲持续时间研究

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Multiphoton imaging (MPI) is an important fluorescence microscopy technique that allows deep tissue and in-vivo imaging with high selectivity. According to theory, two-photon signal is proportional to the product of the peak power and the average power, allowing optimization of key imaging parameters of the excitation laser, such as average power,repetition rate and pulse duration. Recent progress in compact ultrafast lasers including femtosecond fiber lasers and optically pumped semiconductor lasers makes direct control of these parameters possible. In order to investigate the optimum laser parameters for two photon imaging we experimentally study the effects of repetition rate between 2.85 and 90 MHz and pulse duration between 336 fs and 3.5 ps on two photon signal in SYTOX Green labeled mouse intestine sections at 1030 nm. We found that the optimum repetition rate for this sample is in the range 20 - 40 MHz,depending on average power, and that the pulse duration has no effect on the MPI signal provided that the average power can be adjusted to keep the product of average and peak power constant.
机译:多光子成像(MPI)是一项重要的荧光显微镜技术,可对深层组织和体内进行高选择性成像。根据理论,双光子信号与峰值功率和平均功率的乘积成比例,从而可以优化激发激光器的关键成像参数,例如平均功率,重复率和脉冲持续时间。包括飞秒光纤激光器和光泵浦半导体激光器在内的紧凑型超快激光器的最新进展使得直接控制这些参数成为可能。为了研究两个光子成像的最佳激光参数,我们实验性地研究了SYTOX Green标记的小鼠小肠切片在1030 nm处,重复频率在2.85和90 MHz之间以及脉冲持续时间在336 fs和3.5 ps之间对两个光子信号的影响。我们发现,此样本的最佳重复频率在20-40 MHz范围内,具体取决于平均功率,并且只要可以调整平均功率以保持平均乘积,脉冲持续时间就不会对MPI信号产生影响。和峰值功率常数。

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