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Detection of nanoparticles accumulation in biological tissues by optical coherence tomography in vivo

机译:体内光学相干层析成像技术检测生物组织中纳米颗粒的积累

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Dynamics of nanoparticles penetrating and accumulating in healthy (skin) and pathologically altered (tumor) tissue was investigated in vivo by the method of optical coherence tomography (OCT). Gold nanoshells having the size of 130/15 nm and titanium dioxide nanoparticles 40-100 nm in size were studied. Nanoparticles accumulation in biotissue was accompanied by the change of optical effects in OCT images. Continuous OCT monitoring of the process of nanoparticles penetration into skin showed that optical effects appeared 30 minutes after application of nanoparticles on the surface; maximal effect of nanoparticles accumulation in the skin was recorded in the observation period of 1.5-5 hours.rnNanoparticles accumulation in neoplastic tissue at passive delivery was studied in vivo. Accumulation maximum was 4-6 hours after intravenous introduction.rnThe transmission electron microscopy technique confirmed accumulation of nanoparticles in biotissues.
机译:通过光学相干层析成像(OCT)方法,体内研究了纳米粒子在健康(皮肤)和病理改变(肿瘤)组织中的渗透和蓄积动力学。研究了尺寸为130/15 nm的金纳米壳和尺寸为40-100 nm的二氧化钛纳米颗粒。纳米颗粒在生物组织中的积累伴随着OCT图像中光学效应的变化。连续的OCT监测纳米粒子渗透到皮肤的过程表明,将纳米粒子应用到表面后30分钟便出现了光学效应。在观察到的1.5-5小时内记录了纳米颗粒在皮肤中的最大积累作用。研究了体内被动递送时肿瘤组织中纳米颗粒的积累。静脉内引入后最大积累时间为4-6小时。透射电子显微镜技术证实了纳米颗粒在生物组织中的积累。

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