Isolation and Characterization of GSTs Gene Homology Sequences from Agropyron mongolicum Keng

机译：蒙古车前草GSTs基因同源序列的分离与鉴定

获取原文

获取原文并翻译 | 示例

页面导航

摘要
著录项
相似文献
相关主题

摘要

In this study, GSTs gene homology fragment from Mongolian wheatgrass(Agropyron mongolicum Keng) was isolated using homologybased method, one partials gene sequences have been obtained by reverse transcription polymerase chain reaction (RT-PCR). sequence analysis using Software of DNAman and DNAuser etc, and showed that the cDNA sequences was 344 base pairs, encoding 62 amino acids. The partial gene had C-terminal conserved domains of substrate binding pocket (H-site) of GSTs superfamily. Homology comparison with GSTs gene amino acid sequences in other plants showed that it was 91% identical to 19E50 of wheat, 90% to pk0078 and 88% to gstf6. It was named as MwGSTs.

机译：本研究采用基于同源性的方法分离了蒙古小麦草（Ggropyron mongolicum Keng）的GSTs基因同源片段，通过逆转录聚合酶链反应（RT-PCR）获得了部分基因序列。用DNAman和DNAuser等软件进行序列分析，发现cDNA序列为344个碱基对，编码62个氨基酸。该部分基因具有GST超家族的底物结合口袋（H位点）的C端保守结构域。与其他植物中GSTs基因氨基酸序列的同源性比较表明，它与小麦的19E50具有91％的同一性，与pk0078具有90％的同源性，与gstf6具有88％的同源性。它被称为MwGST。

著录项

来源
《New paradigm for diversity of forage production in the east Asian region》|2009年|p.134-135|共2页
会议地点 Seoul(KR)
作者
Yan Zhao; Jin-feng Yun; De-Fu Liu;
展开▼
作者单位

College of Environment, Inner Mongolia Agricultural University, Huhhot, 010018, China Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Huhhot, 010031,China;

College of Environment, Inner Mongolia Agricultural University, Huhhot, 010018, China;

College of Environment, Inner Mongolia Agricultural University, Huhhot, 010018, China;

展开▼
会议组织
原文格式 PDF
正文语种 eng
中图分类饲料作物、牧草;
关键词
Agropyron mongolicum Keng; GSTs; Clone; Homology sequence;

机译：蒙古农杆菌； GSTs；克隆；同源序列;

相似文献

外文文献
中文文献
专利

1. Molecular cloning and characterization of a group 3 LEA gene from Agropyron mongolicum Keng [J] . Yan Zhao, Jinfeng Yun, Fengmin Shi, African Journal of Biotechnology . 2010,第37期

机译：蒙古车前草3族LEA基因的分子克隆与鉴定
2. Transcriptomic and proteomic analyses of drought responsive genes and proteins in Agropyron mongolicum Keng [J] . Yan Zhao, Cuiping Gao, Fengling Shi, Current Plant Biology . 2018,第4期

机译：蒙古沙冰农干旱响应基因和蛋白质的转录组和蛋白质组学分析
3. Cloning and Expression Analysis of AP2/EREBP Transcription Factor Gene (MwAP2/EREBP) in Agropyron mongolicum Keng [J] . Ao Te-Gen-Bai-Yin, Gao Li-Jie, Wang Li-Cheng, Nanoscience and Nanotechnology Letters . 2017,第12期

机译：AP2 / EREBP转录因子基因（MWAP2 / EREBP）在Agropyron Mongolicum Keng的克隆及表达分析
4. Isolation and Characterization of GSTs Gene Homology Sequences from Agropyron mongolicum Keng [C] . Yan Zhao, Jin-feng Yun, De-Fu Liu Korea-China-Japan joint symposium on grassland agriculture and livestock production . 2009

机译：AGRODYRON MONGOLICUM Keng的GSTS基因同源序列的分离与表征
5. Isolation and characterization of a triosephosphate isomerase gene and a root-specific gene from rice, and analysis of their regulatory sequences in transgenic tobacco and rice. [D] . Xu, Yong. 1993

机译：水稻中的磷酸三糖异构酶基因和根特异性基因的分离，鉴定，以及在转基因烟草和水稻中的调控序列分析。
6. Isolation and characterization of human DNA fragments with nucleotide sequence homologies with the simian virus 40 regulatory region. [O] . S E Conrad, M R Botchan 1982

机译：人DNA片段的分离和特征与猿猴病毒40调控区的核苷酸序列同源性。
7. Cloning and expression analysis of cysteine protease gene (MwCP) in Agropyron mongolicum Keng [O] . T.G.B.Y. Ao, M.L. Lang, Y.Q. Li, 2016

机译：Agropyron Mongolicum keng中半胱氨酸蛋白酶基因（MWCP）的克隆及表达分析
8. Molecular Cloning and Sequence Analysis of the Sta58 Major Antigen Gene of Rickettsia tsutsugamushi: Sequence Homology and Antigenic Comparison of Sta58 to the 60-Kilodalton Family of Stress Proteins. [R] . Stover, C. K., Marana, D. P., Dasch, G. A., 1990

机译：恙虫病立克次体sta58主要抗原基因的克隆与序列分析：sta58与60-Kilodalton家系应激蛋白的序列同源性和抗原性比较。

Isolation and Characterization of GSTs Gene Homology Sequences from Agropyron mongolicum Keng

摘要

著录项

相似文献

相关主题

期刊订阅