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首页> 外文期刊>African Journal of Biotechnology >Molecular cloning and characterization of a group 3 LEA gene from Agropyron mongolicum Keng
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Molecular cloning and characterization of a group 3 LEA gene from Agropyron mongolicum Keng

机译：蒙古车前草3族LEA基因的分子克隆与鉴定

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摘要

Late embryogenesis-abundant (LEA) protein is one of the components involved in desiccation tolerance (DT) by maintaining cellular structures in the dry state. In this study, a member of the group 3 LEA,?MwLEA1, was cloned from Mongolian wheatgrass (Agropyron mongolium?Keng) based on a homologous sequence from wheat (Triticum aestivum?L.). Its full-length cDNA sequence was 705 bp, encoding a protein of 187 amino acids. The amino acid sequence comparison revealed its high homology with LEA proteins from other plant species. The deduced?MwLEA1protein had five repeat 11-amino-acid motifs, with a molecular weight of 19.4 kDa and a theoretical isoelectric point of 8.8. Subcellular localization indicated that theMwLEA1?was localized in the nucleus of the onion epithelial cell. Under water stress conditions,?MwLEA1?exhibited different expression levels, which was higher in root and shoot but lowest in leaf. The expression profiling under different stresses indicated that?MwLEA1?played roles in responses to water, salt stresses as well as abscisic acid (ABA) regulation. The gene of?MwLEA1?was transformed into tobaccos by?Agrobacterium tumefaciens-mediated method. Eleven regenerated plants were analyzed by polymerase chain reaction (PCR) and southern blotting, and 6 of them were proved to be transgenic plants.
机译：通过使细胞结构保持在干燥状态，晚期胚胎发生丰富（LEA）蛋白是参与干燥耐性（DT）的成分之一。在这项研究中，根据小麦（Triticum aestivum？L。）的同源序列，从蒙古小麦草（Agropyron mongolium？Keng）克隆了第3组LEA的成员MwLEA1。它的全长cDNA序列为705 bp，编码187个氨基酸。氨基酸序列比较揭示了它与来自其他植物物种的LEA蛋白高度同源。推导的βMwLEA1蛋白具有五个重复的11个氨基酸基序，分子量为19.4kDa，理论等电点为8.8。亚细胞定位表明MwLEA1α定位在洋葱上皮细胞的细胞核中。在水分胁迫条件下，？MwLEA1？表现出不同的表达水平，其在根和芽中较高，而在叶片中最低。在不同压力下的表达谱分析表明，MwLEA1在水，盐胁迫以及脱落酸（ABA）调节中起着作用。用根癌农杆菌介导的方法将“ MwLEA1”基因转化成烟草。通过聚合酶链反应（PCR）和Southern印迹分析了11株再生植物，其中6株被证明是转基因植物。

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