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首页> 外文会议>New Technologies in Non-wood Fiber Pulping and Papermaking >BIOCHEMICAL CHARACTERIZATION OF XYLANASES PRODUCED BY PENICILLIUM SP. ZCF57 AND ITS APPLICATION IN BIOBLEACHING OF WHEAT STRAW PULP
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BIOCHEMICAL CHARACTERIZATION OF XYLANASES PRODUCED BY PENICILLIUM SP. ZCF57 AND ITS APPLICATION IN BIOBLEACHING OF WHEAT STRAW PULP

机译：青霉菌SP产生的木聚糖酶的生物化学表征ZCF57及其在麦草浆生物降解中的应用。

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摘要

A xylanase produced by Penicillium sp. ZCF57 and its two pure enzymes Ⅺ and Ⅻ were characterized in this paper, and its potential application in wheat straw chemical pulp biobleaching was evaluated. The optimum pH and temperature of crude xylanase from ZCF57 was 7.0 and 55 ℃, respectively. It was stable in the range of pH 5.0～10.0 and below 45 ℃. Some of transition metal ions, such as Fe~(2+), Mn~(2+) and Mg~(2+) could increase xylanase activity of ZCF57 dramatically. As far as the enzyme activity was concerned, the optimum temperature and pH value was 55 ℃ and 7.0 for the enzyme Ⅺ, and 50℃ and 6.0 for the enzyme Ⅻ, respectively. It was stable in the condition of below 45℃ and pH 6.0～9.0 for enzyme Ⅺ, but of below 50℃ and pH 5.0～8.0 for enzyme Ⅻ. Biobleaching effectiveness of the crude enzyme, the pure enzyme Ⅺ and Ⅻ were also researched by using enzymes for pretreating wheat straw chemical pulp. The brightness and strength properties of pulp, and the reducing sugars, the lignin-derived compounds and chromophoric materials released in filtrates by the enzymes were analyzed. The results indicated that the xylanase has a good potential application in biobleaching of pulp.
机译：由Penicillium sp。产生的木聚糖酶。本文对ZCF57及其两个纯酶Ⅺ和characterized进行了表征，并评估了其在小麦秸秆化学纸浆生物漂白中的潜在应用。 ZCF57粗木聚糖酶的最适pH和温度分别为7.0和55℃。在pH 5.0〜10.0及45℃以下均稳定。 Fe〜（2 +），Mn〜（2+）和Mg〜（2+）等过渡金属离子可以显着提高ZCF57的木聚糖酶活性。就酶的活性而言，酶Ⅺ的最佳温度和pH值分别为55℃和7.0，酶Ⅻ的最佳温度和pH值分别为50℃和6.0。酶45在45℃以下，pH 6.0〜9.0的条件下稳定，酶enzyme在50℃以下，pH 5.0〜8.0的条件下稳定。还通过使用酶对小麦秸秆化学纸浆进行预处理，研究了粗酶，纯酶Ⅺ和Ⅻ的生物漂白效果。分析了纸浆的亮度和强度特性，以及酶在滤液中释放的还原糖，木质素衍生的化合物和发色物质。结果表明，木聚糖酶在纸浆的生物漂白中具有良好的潜在应用。

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