• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Optical Investigations of Cells In Vitro and In Vivo >Mapping the polarity for T-cell activation with an optical trap,
【24h】

Mapping the polarity for T-cell activation with an optical trap,

机译：用光阱映射T细胞激活的极性，

获取原文
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract: In the body, individual T helper lymphocytes need to be activated first by physical contact with antigen-presenting cells (APC). T-cell contact with APCs initiates an activation cascade which includes an increase in T-cell intracellular calcium and leads to T-cell proliferation and differentiation. We have combined fluroescence spectroscopy and imaging with optical manipulation to investigate the physical properties of T-cell activation. We study cell-cell contact requirements for T-cell activation using optical tweezers to control the orientation of T-cell/APC pairs and fluorescence microscopy to measure the subsequent T-cell intracellular calcium level ($LB@Ca$+2$PLU$/$RB$-i$/) response. APCs or beads coated with antibodies to the T-cell receptor are trapped with a near- infrared titanium-sapphire laser and placed at different locations along the T-cell, which has a polarized appearance defined by the shape and direction of crawling. T cells which are presented with antigen at the leading edge have a higher probability of responding and a shorter latency of response than those contacting APCs or beads with their trailing end. Alterations in antibody density and bead size are used to determine the spatial requirements for T cell activation and the minimum number of receptors which must be engaged in order to transmit a positive signal. !0
机译：摘要：在体内，首先需要通过与抗原呈递细胞（APC）物理接触来激活单个T辅助淋巴细胞。 T细胞与APC接触会启动一个激活级联反应，其中包括T细胞胞内钙的增加并导致T细胞增殖和分化。我们将荧光光谱和成像与光学操作相结合，以研究T细胞活化的物理特性。我们使用光镊控制T细胞/ APC对的方向和荧光显微镜研究随后T细胞胞内钙水平（$ LB @ Ca $ + 2 $ PLU $ / $ RB $ -i $ /）响应。用近红外钛-蓝宝石激光器捕获涂有T细胞受体抗体的APC或珠子，并将其放置在沿T细胞的不同位置，该表面具有由爬行的形状和方向定义的极化外观。与那些在末端接触APC或珠子的T细胞相比，在前缘带有抗原的T细胞具有更高的反应可能性和更短的反应潜伏期。抗体密度和珠大小的变化可用于确定T细胞激活的空间要求以及为传递阳性信号而必须接合的最小受体数量。 ！0

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号