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Mapping the polarity for T-cell activation with an optical trap

机译：用光阱映射T细胞激活的极性

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Abstract: In the body, individual T helper lymphocytes need to beactivated first by physical contact withantigen-presenting cells (APC). T-cell contact withAPCs initiates an activation cascade which includes anincrease in T-cell intracellular calcium and leads toT-cell proliferation and differentiation. We havecombined fluroescence spectroscopy and imaging withoptical manipulation to investigate the physicalproperties of T-cell activation. We study cell-cellcontact requirements for T-cell activation usingoptical tweezers to control the orientation ofT-cell/APC pairs and fluorescence microscopy to measurethe subsequent T-cell intracellular calcium level($LB@Ca$+2$PLU$/$RB$-i$/) response. APCs or beadscoated with antibodies to the T-cell receptor aretrapped with a near- infrared titanium-sapphire laserand placed at different locations along the T-cell,which has a polarized appearance defined by the shapeand direction of crawling. T cells which are presentedwith antigen at the leading edge have a higherprobability of responding and a shorter latency ofresponse than those contacting APCs or beads with theirtrailing end. Alterations in antibody density and beadsize are used to determine the spatial requirements forT cell activation and the minimum number of receptorswhich must be engaged in order to transmit a positivesignal. !0
机译：摘要：在体内，首先需要通过与抗原呈递细胞（APC）物理接触来激活单个T辅助淋巴细胞。 T细胞与APC的接触会启动一个激活级联反应，其中包括T细胞内钙的增加并导致T细胞增殖和分化。我们将荧光光谱和光学操纵成像相结合，以研究T细胞活化的物理特性。我们研究了使用光镊控制T细胞/ APC对的方向和荧光显微镜测量随后的T细胞胞内钙水平（$ LB @ Ca $ + 2 $ PLU $ / $ RB $）对T细胞活化的细胞接触要求。 -i $ /）响应。用近红外钛-蓝宝石激光器捕获涂有T细胞受体抗体的APC或珠子，并将其放置在沿T细胞的不同位置，该表面具有由爬行的形状和方向定义的极化外观。与在末端带有抗原的APC或珠子接触的T细胞相比，在抗原前沿出现的T细胞具有更高的响应概率和更短的响应潜伏期。抗体密度和珠粒大小的变化用于确定T细胞活化的空间要求以及为传递正信号而必须接合的最小受体数目。 ！0

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