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Angular domain optical projection tomography in turbid media

机译:混浊介质中的角域光学投影层析成像

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Most high-resolution optical tomography techniques employ coherence domain or time domain methodologies to capture non-scattered photons in turbid media. Angular Domain Optical Projection Tomography (ADOPT) uses an angular filter array (AFA) to observe photons that propagate through a specimen with small angular deviation. We constructed an ADOPT system consisting of an AFA micro-machined silicon micro-tunnel array with each micro-tunnel 60 μm wide, 60 μm high, 10 mm long, and separated by 5 μm thick walls. The range of acceptance angles was 0° to 0.5°. The system also included an 808 nm CW diode laser, beam shaping optics, a sample cuvette, a Keplerian lens system, and a CMOS camera. Testing was performed with a target consisting of two graphite rods (0.9 mm diameter) suspended in the cuvette by a rotation stage. The target was placed in a manner that the line of laser light was perpendicular to the long axis of the rods. A multitude of projections were collected at increments of 1.8° and compiled into a sinogram. A transverse image was reconstructed from the sinogram using filtered backprojection. The sub-millimeter targets embedded in the 2 cm thick scattering medium (reduced scattering coefficient ≤ 2.4 cm~(-1)) were discernable in both the sinograms and the reconstructed images. The results suggest that ADOPT may be a useful technique for tomographic imaging of thick biological specimens (i.e. up to 8 mm across).
机译:大多数高分辨率光学层析成像技术采用相干域或时域方法来捕获浑浊介质中的非散射光子。角域光学投影层析成像(ADOPT)使用角滤镜阵列(AFA)观察以小角度偏差传播通过样本的光子。我们构建了一个ADOPT系统,该系统由AFA微机械加工的硅微隧道阵列组成,每个微隧道宽60μm,高60μm,长10 mm,并由5μm厚的壁隔开。接收角的范围是0°至0.5°。该系统还包括一个808 nm CW二极管激光器,光束整形光学器件,一个样品比色杯,一个Keplerian透镜系统和一个CMOS相机。使用由两个石墨棒(直径0.9毫米)组成的靶子通过旋转台悬挂在比色杯中进行测试。以激光线垂直于棒的长轴的方式放置目标。以1.8°的增量收集大量投影,并将其汇总成正弦图。使用滤波后的投影从正弦图重建横向图像。在正弦图和重建图像中都可以分辨出嵌入2 cm厚散射介质(降低的散射系数≤2.4 cm〜(-1))中的亚毫米目标。结果表明ADOPT可能是对厚厚的生物标本(即最大8毫米宽)进行断层成像的有用技术。

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