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首页> 外文会议>Optics in health care and biomedical optics VI >Quantitative changes of collagen in human normal breast tissue and invasive ductal carcinoma using nonlinear optical microscopy
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Quantitative changes of collagen in human normal breast tissue and invasive ductal carcinoma using nonlinear optical microscopy

机译：非线性光学显微镜观察人正常乳腺组织和浸润性导管癌中胶原蛋白的定量变化

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摘要

Multiphoton microscopy (MPM) imaging of collagen plays a key role in noninvasive diagnosis of human tissue. During the experiment, we observed an interesting phenomenon which two-photon excited fluorescence (TPEF) signal of collagen in human invasive ductal carcinoma of breast tissue becomes much weaker than the normal breast tissue, but the second harmonic generation (SHG) signal of collagen does not get an obvious change . In order to explain the phenomena,this paper emphasizes on the intensity of TPEF and SHG signal from collagen in human invasive ductal carcinoma of breast tissues and normal breast tissue. Further, we respectively obtain the intensity spectral information from collagen in the above two tissues with all parameter unaltered. Our quantitative results show that the intensity of TPEF from collagen in human invasive ductal carcinoma of breast tissue is much lower than the intensity of TPEF from collagen in normal breast tissue. According to the theoretic analysis, it was concluded that the intensity of TPEF declined due to the reduction of the quantum yield when the collagen was intruded by cancer cells. However, the invasion of cancer cells has no effect on decisive factor of SHG Our theoretical analysis brings more detailed information about intensity of SHG and TPEF from collagen in the above two tissues.
机译：胶原蛋白的多光子显微镜（MPM）成像在人类组织的非侵入性诊断中起着关键作用。在实验过程中，我们观察到一个有趣的现象：人乳腺浸润性导管癌中胶原蛋白的双光子激发荧光（TPEF）信号变得比正常乳腺组织弱得多，但是胶原蛋白的二次谐波（SHG）信号确实没有明显的变化。为了解释这种现象，本文重点研究了人乳腺浸润性导管癌和正常乳腺组织中胶原蛋白产生的TPEF和SHG信号的强度。此外，我们分别从上述两个组织中的胶原蛋白获得强度光谱信息，而所有参数均未改变。我们的定量结果表明，人乳腺浸润性导管癌中胶原蛋白产生的TPEF的强度远低于正常乳腺组织中胶原产生的TPEF的强度。根据理论分析，可以得出结论，当胶原蛋白被癌细胞侵袭时，TPEF的强度下降是由于量子产率的降低。然而，癌细胞的入侵对SHG的决定因素没有影响。我们的理论分析为上述两种组织中胶原蛋白对SHG和TPEF的强度提供了更详细的信息。

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  • 来源
    《Optics in health care and biomedical optics VI》|2014年|926827.1-926827.8|共8页
  • 会议地点 Beijing(CN)
  • 作者

    Weiqiang Li; Yan Wu; Yuane Lian; Fangmeng Fu; Chuan Wang; Shuangmu Zhuo; Jianxin Chen;

  • 作者单位

    Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China;

    Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China,School of Science, Jimei University, Xiamen, 361021, China;

    Department of Pathology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, 350001, China;

    Department of Breast Surgery, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, 350001, China;

    Department of Breast Surgery, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, 350001, China;

    Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China;

    Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fujian Normal University, Fuzhou, 350007, China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    multiphoton microscopy; two-photon excited fluorescence; second harmonic generation; collagen; breast tissue; quantum yield;

    机译：多光子显微镜双光子激发荧光；二次谐波产生；胶原;乳房组织；量子产率;

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