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首页> 外文会议>Photonics, devices, and systems V >Raman microspectroscopy of algal lipid bodies: β-carotene as a volume sensor
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Raman microspectroscopy of algal lipid bodies: β-carotene as a volume sensor

机译：藻类脂质体的拉曼光谱：β-胡萝卜素作为体积传感器

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摘要

Advanced optical instruments are useful for analysis and manipulation of individual living cells and their internal structures. We have employed Raman microspectroscopic analysis for assessment of algal lipid body (LB) volume in vivo. Some algae contain p-carotene in high amounts in their LBs, including strains which are considered useful in biotechnology for lipid and pigment production. We have detected proportionality between the Raman vibrations of p-carotene and the LB volume. This finding may allow fast acquisition of LB volume approximation valuable e.g. for Raman microspectroscopy assisted cell sorting. We combine optical manipulation and analysis on a microfluidic platform in order to achieve fast, effective, and non-invasive sorting based on spectroscopic features of the individual living cells. The resultant apparatus could find its use in demanding biotechnological applications such as selection of rare natural mutants or artificially modified cells resulting from genetic manipulations.
机译：先进的光学仪器可用于分析和处理单个活细胞及其内部结构。我们已经采用拉曼光谱分析来评估体内藻类脂质体（LB）的体积。一些藻类在其LB中含有大量的对-胡萝卜素，包括被认为在生物技术中用于生产脂质和色素的菌株。我们已经检测到对胡萝卜素的拉曼振动与LB体积之间的比例关系。该发现可以允许快速获取有价值的例如LB的体积近似值。用于拉曼光谱辅助细胞分选。我们在微流控平台上结合了光学操作和分析功能，以便基于单个活细胞的光谱特征实现快速，有效和无创的分选。所得设备可以在苛刻的生物技术应用中找到其用途，例如选择罕见的自然突变体或通过基因操作产生的人工修饰的细胞。

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  • 来源
    《Photonics, devices, and systems V》|2011年|p.83060L.1-83060L.7|共7页
  • 会议地点 Prague(CS)
  • 作者

    Zdenek Pilat; Silvie Bernatova; Jan Jezek; Mojmir Sery; Ota Sarnek; Pavel Zemanek; Ladislav Nedbal; Martin Trtilek;

  • 作者单位

    Institute of Scientific Instruments of the AS CR, v.v.i., Academy of Sciences of the Czech Republic,Kralovopolska 147, 612 64 Brno, Czech Republic;

    Institute of Scientific Instruments of the AS CR, v.v.i., Academy of Sciences of the Czech Republic,Kralovopolska 147, 612 64 Brno, Czech Republic;

    Institute of Scientific Instruments of the AS CR, v.v.i., Academy of Sciences of the Czech Republic,Kralovopolska 147, 612 64 Brno, Czech Republic;

    Institute of Scientific Instruments of the AS CR, v.v.i., Academy of Sciences of the Czech Republic,Kralovopolska 147, 612 64 Brno, Czech Republic;

    Institute of Scientific Instruments of the AS CR, v.v.i., Academy of Sciences of the Czech Republic,Kralovopolska 147, 612 64 Brno, Czech Republic;

    Institute of Scientific Instruments of the AS CR, v.v.i., Academy of Sciences of the Czech Republic,Kralovopolska 147, 612 64 Brno, Czech Republic;

    Global Change Research Centre of the AS CR, v.v.i., Academy of Sciences of the Czech Republic,Belidla 986/4a, 603 00 Brno, Czech Republic;

    Photon Systems Instruments, Drasov 470, 664 24 Drasov, Czech Republic;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 工程光学;
  • 关键词

    raman microspectroscopy; microalgae; trachydiscus minutus; biotechnology; optical tweezers; microfluidics;

    机译：拉曼光谱微藻细曲霉生物技术光学镊子；微流体;

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