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首页> 外文会议>Photons Plus Ultrasound: Imaging and Sensing 2006; Progress in Biomedical Optics and Imaging; vol.7, no.9 >Photoacoustic Spectroscopic Imaging of Intra-Tumor Heterogeneity and Molecular Identification
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Photoacoustic Spectroscopic Imaging of Intra-Tumor Heterogeneity and Molecular Identification

机译：肿瘤内异质性的光声光谱成像和分子鉴定

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Purpose. To evaluate photoacoustic spectroscopy as a potential imaging modality capable of measuring intra-tumor heterogeneity and spectral features associated with hemoglobin and the molecular probe indocyanine green (ICG). Material and Methods. Immune deficient mice were injected with wildtype and VEGF enhanced MCF-7 breast cancer cells or SKOV3x ovarian cancer cells, which were allowed to grow to a size of 6-12 mm in diameter. Two mice were imaged alive and after euthanasia for (oxy/deoxy)-hemoglobin content. A 0.4 mL volume of 1 μg/mL concentration of ICG was injected into the tail veins of two mice prior to imaging using the photoacoustic computed tomography (PCT) spectrometer (Optosonics, Inc., Indianapolis, IN 46202) scanner. Mouse images were acquired for wavelengths spanning 700-920 nm, after which the major organs were excised, and similarly imaged. A histological study was performed by sectioning the organ and optically imaging the fluorescence distribution. Results. Calibration of PCT-spectroscopy with different samples of oxygenated blood reproduced a hemoglobin dissociation curve consistent with empirical formula with an average error of 5.6%. In vivo PCT determination of SaO_2 levels within the tumor vascular was measurably tracked, and spatially correlated to the periphery of the tumor. Statistical and systematic errors associated with hypoxia were estimated to be 10 and 13%, respectively. Measured ICG concentrations determined by contrast-differential PCT images in excised organs (tumor, liver) were approximately 0.8 μg/mL, consistent with fluorescent histological results. Also, the difference in the ratio of ICG concentration in the gall bladder-to-vasculature between the mice was consistent with excretion times between the two mice. Conclusion. PCT spectroscopic imaging has shown to be a noninvasive modality capable of imaging intra-tumor heterogeneity of (oxy/deoxy)-hemoglobin and ICG in vivo, with an estimated error in SaO_2 at 17% and in ICG at 0.8 μg/mL in excised tissue. Ongoing development of spectroscopic analysis techniques, probe development, and calibration techniques are being developed to improve sensitivity to both exogenous molecular probes and (oxy/deoxy)-hemoglobin fraction.
机译：目的。评价光声光谱法作为一种潜在的成像方式，能够测量与血红蛋白和分子探针吲哚菁绿（ICG）相关的肿瘤内异质性和光谱特征。材料与方法。向免疫缺陷的小鼠注射野生型和VEGF增强的MCF-7乳腺癌细胞或SKOV3x卵巢癌细胞，使其生长至直径6-12 mm。对两只小鼠进行活体成像，并在安乐死后对（氧/脱氧）-血红蛋白含量进行成像。在使用光声计算机断层摄影（PCT）光谱仪（Optosonics，Inc.，Indianapolis，IN 46202）扫描仪进行成像之前，将0.4 mL体积的1μg/ mL浓度的ICG注入两只小鼠的尾静脉。采集小鼠图像的波长为700-920 nm，然后切除主要器官，并进行类似成像。通过切片器官并光学成像荧光分布进行组织学研究。结果。用不同的含氧血液样本对PCT光谱进行校准，可再现与经验公式一致的血红蛋白解离曲线，平均误差为5.6％。体内PCT测定肿瘤血管内SaO_2的水平是可以测量的，并且在空间上与肿瘤的周围相关。与缺氧有关的统计和系统误差估计分别为10％和13％。通过对比差分PCT图像在切除的器官（肿瘤，肝脏）中测得的ICG浓度约为0.8μg/ mL，与荧光组织学结果一致。同样，小鼠之间胆囊与脉管系统中ICG浓度之比的差异与两只小鼠之间的排泄时间一致。结论。 PCT光谱成像已被证明是一种能够在体内对（氧/脱氧）-血红蛋白和ICG进行肿瘤内异质性成像的非侵入性方式，在切除的组织中SaO_2的估计误差为17％，ICG的估计误差为0.8μg/ mL 。正在进行光谱分析技术，探针开发和校准技术的开发，以提高对外源分子探针和（氧/脱氧）血红蛋白组分的敏感性。

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