• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Photosynthesis research for food, fuel and future >The Periplasmic Carbonic Anhydrase, CAH1, is Absent in the Sequenced Chlamydomonas Reinhardtii Strain, CC-503
【24h】

The Periplasmic Carbonic Anhydrase, CAH1, is Absent in the Sequenced Chlamydomonas Reinhardtii Strain, CC-503

机译：序列衣藻莱茵菌CC-503中缺少周质碳酸酐酶CAH1。

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Chlamydomonas reinhardtii strain CC-503 (cw-92, mt+), is a cell wall deficient strain that was used in the C. reinhardtii genome sequencing project. During the course of working with this strain, it was discovered that it lacks the periplasmic carbonic anhydrase, CAH1. The protein is undetectable on immunoblots probed with a polyclonal CAH1 antibody although other Carbon Concentrating Mechanism (CCM) proteins are found to be present in the expected amounts. However, the amount of CAH1 mRNA in CC-503, analyzed by quantitative RT-PCR (qRT-PCR), shows no significant difference when compared to other wildtype strains, both walled and cell wall deficient. CC-503 shows reduced growth at lower pH conditions but no significant deficiency in the induction of the CCM. By serving as a natural CAH1 mutant, this strain could be used in experiments investigating the importance of this carbonic anhydrase in C. reinhardtii.
机译：莱茵衣藻CC-503株（cw-92，mt +）是一种细胞壁缺陷株，用于莱茵衣藻基因组测序项目。在处理该菌株的过程中，发现它缺乏周质碳酸酐酶CAH1。该蛋白在用多克隆CAH1抗体探测的免疫印迹上无法检测到，尽管发现其他碳浓缩机制（CCM）蛋白也以预期的量存在。然而，通过定量RT-PCR（qRT-PCR）分析，CC-503中CAH1 mRNA的量与壁和细胞壁均缺乏的其他野生型菌株相比，无显着差异。 CC-503在较低的pH条件下显示出生长减少，但在诱导CCM方面没有明显的缺陷。通过用作天然CAH1突变体，该菌株可用于研究这种碳酸酐酶在莱茵衣藻中的重要性的实验。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号