• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Plasma for bio-decontamination, medicine and food security >Non-thermal Atmospheric Plasma Treatment for Deactivation of Oral Bacteria and Improvement of Dental Composite Restoration
【24h】

Non-thermal Atmospheric Plasma Treatment for Deactivation of Oral Bacteria and Improvement of Dental Composite Restoration

机译：非热常压等离子体治疗口腔细菌失活和改善牙科复合修复

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This paper reviews our recent research results of using non-thermal atmospheric plasmas for oral bacterial deactivation and for composite restoration improvement. Oral bacteria of Streptococcus mutans (S. mutans) and Lactobacillus acidophilus (L. acidophilus) with an initial bacterial population density between 1.0 × 10~8 and 5.0 × 10~8 cfu/ml were seeded on various media and their survivability with plasma exposure was examined. The plasma exposure time for a 99.9999% cell reduction was less than 15 s for S. mutans and within 5 min for L. acidophilus. To evaluate the dentin/composite interfacial bonding, extracted unerupted human third molars were used by removing the crowns and etching the exposed dentin surfaces with 35% phosphoric acid gel. After dental composite application and light curing, the teeth were then sectioned into micro-bars as the specimens for microtensile test. Student Newman Keuls (SNK) tests showed that the bonding strength of the composite restoration to peripheral dentin was significantly increased (by 64%) after 30 s plasma treatment of the dentin surfaces. These findings indicated that non-thermal atmospheric plasma technology is very promising for dental clinical applications. Polymethacrylate-based dental composites have received widespread clinical acceptance as alternative restorative materials to dental amalgam amid concern regarding the potential health risks associated with mercury released from dental amalgam. Polymer-based dental composites can be easily adapted to a wide variety of direct placement applications, and are aesthetically appealing. Dental composite restorations, however, have much shorter longevity when compared to amalgam restorations [1-4]. An 8-year clinical study found that the failure rate for posterior composite restorations was two to three times higher than amalgam restorations [5]. Many other studies reported very similar results [6-8]. The reduced longevity of dental composite restorations is troubling as the removal of these restorations can lead to extensive loss of sound tooth structure and eventual loss of the teeth after two or three restoration replacements [9]. Extensive clinical and laboratory studies indicate that the reduced longevity of dental composite restoration most often resulted from the interface failure (i.e., adhesive bonding failure of the composite restoration to the surrounding tooth structure) [10-15]. The interface failure results in the separation of the composite restoration from dentin. The resulting gaps lead to marginal staining, sensitivity, and recurrent caries [12, 13, 16, 17], which cause a significant portion of composite restoration removal and replacements [2, 17], and therefore are significant in terms of reducing the longevity of the composite restoration. The interface failures in composite restoration are commonly found at the adhesive/dentin interface [10, 18, 19]. Adhesion between enamel and composite is generally adequate for clinical applications, while adhesive/dentin interface is weak and the interfacial bonding strength deteriorates significantly over time [ 18-21 ]. For example, an in vivo study showed that the mean tensile bond strength values dropped from 28.3 MPa at 24 h to 9.1 MPa at 2-3 years [19]. Unsuccessful dentin bonding also means that there are sites at the tooth restoration interface that are vulnerable to hydrolytic breakdown and susceptible to attack by bacterial enzymes [9]. The improvement in adhesive/dentin interfacial bonding is essential to achieve robust and durable composite restoration with increased longevity. As an effective surface/interface engineering tool, non-thermal gas plasma technique provides a unique opportunity in dentin surface preparation to disinfect oral bacteria and modifying dentin surfaces to significantly strengthen the interface bonding, and thus to increase the longevity of composite restorations. In this paper, we summarized our recent research results on plasma deactivation of oral bacteria and surface treatment of dentin surfaces for interfacial bonding improvement with dental adhesive and composite application.
机译：本文回顾了我们最近使用非热大气等离子体进行口腔细菌灭活和复合修复的研究成果。初始细菌种群密度在1.0×10〜8和5.0×10〜8 cfu / ml之间的变异链球菌和嗜酸乳杆菌的口腔细菌接种到各种培养基上，并通过血浆暴露存活被检查了。对于变形链球菌，细胞减少99.9999％的血浆暴露时间少于15 s，对于嗜酸乳杆菌，其血浆暴露时间少于5分钟。为了评估牙本质/复合物的界面结合力，通过拔出牙冠并用35％的磷酸凝胶蚀刻暴露的牙本质表面，使用提取的未中断的人类第三磨牙。在应用牙科复合材料并进行光固化后，将牙齿切成微条，作为微拉伸试验的样本。 Student Newman Keuls（SNK）测试显示，经过30 s的牙本质表面等离子体处理后，复合修复体与周围牙本质的结合强度显着提高（提高了64％）。这些发现表明，非热大气等离子体技术对于牙科临床应用非常有前途。基于聚甲基丙烯酸酯的牙科复合材料作为牙科用汞合金的替代修复材料已获得广泛的临床认可，这是由于人们担心与牙科用汞合金释放的汞有关的潜在健康风险。聚合物基牙科复合材料可轻松适应各种直接放置应用，并且在美学上具有吸引力。然而，与汞合金修复体相比，牙科复合修复体的寿命要短得多[1-4]。一项为期8年的临床研究发现，后路复合修复的失败率是汞合金修复的2至3倍[5]。许多其他研究报告了非常相似的结果[6-8]。牙齿复合材料修复体寿命的降低令人不安，因为去除这些修复体会导致牙齿健全结构的大量丧失，并在两次或三次修复体置换后最终导致牙齿的丧失[9]。大量的临床和实验室研究表明，牙科复合材料修复体寿命的缩短通常是由于界面失效（即，复合材料修复体与周围牙齿结构的粘合失败）引起的[10-15]。界面破坏导致复合修复体与牙本质分离。产生的间隙会导致边缘染色，敏感度和龋齿复发[12、13、16、17]，这会导致复合修复体的去除和替换[2、17]占很大比例，因此在降低寿命方面很重要复合修复体。复合修复中的界面失效通常在粘合剂/牙本质界面[10、18、19]处发现。牙釉质和复合材料之间的粘合力通常足以满足临床应用，而胶粘剂/牙本质的界面较弱，并且界面粘合强度会随时间而显着降低[18-21]。例如，一项体内研究表明，平均抗拉强度值从24小时的28.3 MPa降至2-3年的9.1 MPa [19]。牙本质粘合不成功还意味着牙齿修复界面上存在易水解分解且易受细菌酶攻击的位点[9]。粘合剂/牙本质界面粘合的改善对于实现耐用性和持久性的复合修复并延长使用寿命至关重要。作为一种有效的表面/界面工程工具，非热气体等离子体技术为牙本质表面制备提供了独特的机会，可对口腔细菌进行消毒并修饰牙本质表面，从而显着增强界面结合力，从而延长复合修复体的使用寿命。在本文中，我们总结了我们最近在口腔细菌的血浆失活和牙本质表面的表面处理方面的研究成果，以改善牙科用粘合剂和复合材料的界面粘结性。

著录项

  • 来源
  • 会议地点 Demanovska Dolina(SK)
  • 作者

    Qing Song Yu; H. Li; A.C. Ritts; B. Yang; M. Chen; L. Hong; C. Xu; X. Yao; Y. Wang;

  • 作者单位

    Center for Surface Science and Plasma Technology, Department of Mechanical and Aerospace Engineering, University of Missouri, E2403D Lafferre Hall, Columbia, MO 65211, USA;

    Center for Surface Science and Plasma Technology, Department of Mechanical and Aerospace Engineering, University of Missouri, E2403D Lafferre Hall, Columbia, MO 65211, USA;

    Center for Surface Science and Plasma Technology, Department of Mechanical and Aerospace Engineering, University of Missouri, E2403D Lafferre Hall, Columbia, MO 65211, USA;

    Center for Surface Science and Plasma Technology, Department of Mechanical and Aerospace Engineering, University of Missouri, E2403D Lafferre Hall, Columbia, MO 65211, USA;

    Nanova Inc., Columbia, MO 65203, USA;

    College of Dentistry, University of Tennessee, Memphis, TN 38136, USA;

    School of Dentistry, University of Missouri-Kansas City, Kansas City, MO 64108, USA;

    School of Dentistry, University of Missouri-Kansas City, Kansas City, MO 64108, USA;

    School of Dentistry, University of Missouri-Kansas City, Kansas City, MO 64108, USA;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物工程学（生物技术）;
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号