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首页> 外文期刊>American journal of dentistry >Effects of atmospheric non-thermal argon/oxygen plasma on biofilm viability and hydrophobicity of oral bacteria
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Effects of atmospheric non-thermal argon/oxygen plasma on biofilm viability and hydrophobicity of oral bacteria

机译：大气非热氩/氧等离子体对口腔细菌生物膜活力和疏水性的影响

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Purpose: To evaluate the bactericidal effects of atmospheric non-thermal argon/oxygen plasma on in vitro oral biofilms constructed from S. mutans and/or S. sanguinis, and the influence of the plasma on the virulence properties of A. oris. Methods: In vitro oral biofilms were constructed in the wells of 48-well plates from S. mutans and/or S. sanguinis. The wells containing constructed biofilms and various amounts of phosphate-buffered saline (PBS) were treated with non thermal argon/oxygen plasma brush for 2 minutes. The methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and Live/Dead assay were used to evaluate the viability of biofilms in those wells after the plasma treatments. Meanwhile, A. oris suspensions were treated with the plasma and then evaluated for their virulence properties by measuring the hydrophobicity and co-aggregation capability of treated A. oris. Results: The MTT assay showed that exposure to non thermal plasma for 2 minutes significantly reduced the viability of bacteria in both single-species and two-species biofilms of S. mutans and S. sanguinis with the reductions of up to 99%. Meanwhile, plasma treatment also altered the hydrophobicity of A. oris, and reduced their capability to co-aggregate with S. sanguinis.
机译：目的：评价大气非热氩/氧等离子体对由S.umentans和/或S. suguinis构建的体外口腔生物膜的杀菌效应以及血浆对A的毒力学性质的影响。方法：在48孔板的孔中，在S. mutans和/或S.Anganguinis中构建体外口腔生物膜。含有构造的生物膜的孔和各种量的磷酸盐缓冲盐水（PBS）用非热氩/氧等离子体刷处理2分钟。使用甲基噻唑二苯基 - 四唑鎓溴化氢（MTT）测定和活/死导在等离子体处理后评估生物膜在那些孔中的活力。同时，通过测量血浆治疗A. Oris悬浮液，然后通过测量治疗A的疏水性和共聚合能力来评估其毒力学性能。结果：MTT测定显示，暴露于非热血浆2分钟，显着降低了细菌在单一物种和S.Ulans和S.anguinis的两种物种生物膜中的活力，降低了高达99％。同时，等离子体处理也改变了A的疏水性，并降低了它们与S. anguinis共骨料的能力。

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