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首页> 外文会议>Proceedings of the 18th congress of the International Society for Mushroom Science >Molecular Diversity of Wild Auricularia polytricha in Guangdong Province Revealed by ISSR Analysis
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Molecular Diversity of Wild Auricularia polytricha in Guangdong Province Revealed by ISSR Analysis

机译：ISSR分析揭示广东省野生木耳的分子多样性

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摘要

Inter-simple sequence repeat (ISSR) markers were used to investigate the genetic diversity of 13 wild Auricularia polytricha strains collected from Guangdong Province and six cultivars from Henan, Fujian, Guangdong and Hainan. Eight primers produced a total of 265 loci in 19 strains, and the percentage of polymorphic loci was 99%. The mean genetic similarity of wild A. polytricha was 75%, and 72. 3% in cultivated strains, suggested the genetic variation of cultivars was slightly higher than that intraregional wild strains. Clustering analysis based on genetic distance revealed the 19 strains could be classified into three distinct groups. Group I composed all the wild strains, Group II included three cultivars from Henan and Guangdong that had a closer relationship with the wild strains, and the group HI comprised the other three cultivars. The results of principal coordinate analysis were in accordance with the neighbor-joining clustering, and the first three most informative coordinate accounted for 74.3% of the variations, indicated a high level of genetic diversity among A. polytricha strains.
机译：使用简单序列间重复序列（ISSR）标记研究了广东省收集的13种野生多毛木耳菌株和河南，福建，广东和海南的6个品种的遗传多样性。 8个引物在19个菌株中产生了总共265个基因座，多态性基因座的百分比为99％。野生曲霉的平均遗传相似性为75％，在栽培菌株中为72. 3％，这表明栽培品种的遗传变异比区域内野生菌株略高。基于遗传距离的聚类分析表明，这19个菌株可以分为三个不同的组。第一组包括所有野生菌株，第二组包括来自河南和广东的三个与野生菌株关系更密切的品种，而第一组包括其他三个品种。主坐标分析的结果与邻居加入的聚类一致，前三个信息最丰富的坐标占变异的74.3％，表明多毛曲霉菌株之间的遗传多样性很高。

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