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首页> 外文期刊>Cryptogamie. Mycologie >Assessment of genetic diversity among wild Auricularia polytricha populations in China using ISSR markers
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Assessment of genetic diversity among wild Auricularia polytricha populations in China using ISSR markers

机译：利用ISSR标记评价中国野生木耳种群的遗传多样性。

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The genetic diversity of Auricularia polytricha among 27 wild populations in China was analyzed by using inter-simple sequence repeat (ISSR) markers. At the species level a total of 509 loci were amplified using eleven ISSR primers with the percentage of polymorphic loci (P) = 100%, Nei's gene diversity (H) = 0.1935, Shannon information index (I) = 0.3274, and total genetic diversity (H _t) = 0.1918. At the population level, P = 35.3%, H = 0.1277 and I = 0.1907 whereas diversity within populations (H _S) is 0.1135. A high degree of genetic differentiation (G _(st) = 0.4081) among populations was detected. This genetic structure is likely due to the combined effects of the limited gene flow (N _m = 0.7251), geographical isolation, and random genetic drift. Clustering analysis revealed that a remarkable geographical relatedness exists among strains within population, whereas no distinct geographical pattern occurs in the genetic variation among populations. Furthermore, clustering analysis did not suggest genetic variation to be correlated with the host association.
机译：利用简单序列重复序列（ISSR）标记分析了中国27个野生种群的多毛耳的遗传多样性。在物种水平上，使用11种ISSR引物扩增了509个基因座，其中多态性基因座（P）= 100％，Nei基因多样性（H）= 0.1935，Shannon信息指数（I）= 0.3274，总遗传多样性（H _t）= 0.1918。在人口水平上，P = 35.3％，H = 0.1277，I = 0.1907，而人口内部的多样性（H_S）为0.1135。在人群中检测到高度的遗传分化（G _（st）= 0.4081）。这种遗传结构可能是由于有限的基因流（N_m = 0.7251），地理隔离和随机遗传漂移的综合作用所致。聚类分析表明，种群内的菌株之间存在显着的地理相关性，而种群间的遗传变异没有明显的地理格局。此外，聚类分析未表明遗传变异与宿主协会相关。

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