Identification of Trichoderma spp. in Mushroom Farms in Serbia

机译：鉴定木霉属。在塞尔维亚的蘑菇农场

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Disease with symptoms resembling green mould was noted in mushroom facilities in Serbia. The aims of this study were to isolate and identify the causal agents of green mould, examine variation of the pathogen as evidenced by morphology of its colonies, using molecular biology techniques and their pathogenic characteristics. Pathogenicity assay was performed on harvested basidiomes of A. bisporus treated with a 3 X 106 conidia/mL of spore suspensions of all tested Trichoderma spp. isolates. Identification of Trichoderma spp. was based on colonial morphology, optimal growth temperature, odor, conidial morphology and conidia length and width. Identification was confirmed using molecular techiques using sequence analyses of ITS1/4 amplicon. Diseased fruiting bodies of A. bisporus with symptoms resembling green mold disease were observed in 14 mushroom units during 2006-2010. Nectrotic lesions and brown, rust coloured spots were observed on caps of fruiting bodies of eleven A. bisporus and one Lentinus edodes mushroom farms from Serbia and one A. bisporus farm from Bosnia and Hezegovina. Samples of compost with large dark areas of dense green sporulation were collected from one mushroom farm in Serbia. Pathogenicity assay on mushroom pilei showed that each of the tested isolates had high virulence level for A. bispours. The isolates were classified according their morphological characteristics as:T. atroviride, T. koningii, T. virens, T. aggressivum f. sp. aggressivum and T. harzianum. The phylogenetic analyses on ITS1/4 amplicons confirmed the differentiation obtained with classical mycological methods.

机译：在塞尔维亚的蘑菇设施中发现了症状类似绿色霉菌的疾病。这项研究的目的是使用分子生物学技术及其致病特征，分离和鉴定绿色霉菌的病原体，检查病原体的变异，如其菌落形态所证明的。对用3 X 106分生孢子/ mL所有测试的木霉属菌种的孢子悬浮液处理的双孢曲霉的收获的basidiomes进行致病性测定。隔离株。鉴定木霉属。根据殖民地形态，最佳生长温度，气味，分生孢子形态和分生孢子的长度和宽度确定。使用ITS1 / 4扩增子的序列分析，通过分子技术确认了鉴定。在2006-2010年期间，在14个蘑菇单位中观察到双孢曲霉的有病子实体出现类似绿色霉菌的症状。在来自塞尔维亚的11个双孢蘑菇和一个香菇蘑菇农场以及来自波斯尼亚和黑塞哥维那的一个双孢蘑菇农场的子实体的顶盖上观察到了芽胞病损害和褐色的锈色斑点。从塞尔维亚的一个蘑菇农场中收集了堆肥的样品，其中有大片深色的密集的绿色孢子。在蘑菇堆上的致病性分析表明，每个测试菌株均对双歧曲霉具有高毒力。分离物根据其形态特征分类为：T。 atroviride，T。koningii，T。virens，T.aggressivum f。 sp。侵略性和T. harzianum。 ITS1 / 4扩增子的系统发育分析证实了经典真菌学方法获得的分化。

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来源
《Proceedings of the 18th congress of the International Society for Mushroom Science》|2012年|370-376|共7页
会议地点 Beijing(CN)
作者
Dejana Kosanovi(c); Ivana Poto(c)nik; Bojan Duduk; Svetlana Milija(s)evi(c)-Mar(c)i(c); Emil Rekanovi(c); Milos Stepanovi(c); Biljana Todorovi(c);
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作者单位

Institute of Pesticides and Environmental Protection, Banatska 31B, Belgrade 11080, Serbia Institute of Botany,Faculty of Biology, University of Belgrade, Takovska 43, Belgrade 11000, Serbia;

Institute of Pesticides and Environmental Protection, Banatska 31B, Belgrade 11080, Serbia;

Institute of Pesticides and Environmental Protection,Banatska 31B, Belgrade 11080, Serbia;

Institute of Pesticides and Environmental Protection, Banatska 31B, Belgrade 11080, Serbia;

Institute of Pesticides and Environmental Protection, Banatska 31B, Belgrade 11080, Serbia;

Institute of Pesticides and Environmental Protection, Banatska 31B, Belgrade 11080, Serbia;

Institute of Pesticides and Environmental Protection, Banatska 31B, Belgrade 11080, Serbia;

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原文格式 PDF
正文语种 eng
中图分类菌类（食用菌）;
关键词
Green Mould; Cultivated Mushroom;

机译：绿色模具；栽培蘑菇;

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专利

1. ISOLATION AND IDENTIFICATION OF BACILLUS SPP. FROM COMPOST MATERIAL, COMPOST AND MUSHROOM CASING SOIL ACTIVE AGAINST TRICHODERMA SPP. [J] . Olja Stanojevi?, Svetlana Milija?evi?-Mar?i?, Ivana Poto?nik, Archives of Biological Sciences . 2016,第4期

机译：芽孢杆菌SPP的分离和鉴定。从堆肥材料，堆肥和蘑菇套管土壤中去除毛滴虫。
2. Fungicide sensitivity of Trichoderma spp. from Agaricus bisporus farms in Serbia [J] . DEJANA KOSANOVIC, IVANA POTOCNIK, JELENA VUKOJEVIC, Journal of Environmental Science and Health. Part B . 2015,第7a9期

机译：木霉菌对杀菌剂的敏感性。来自塞尔维亚的双孢蘑菇农场
3. Trichoderma spp. - The Cause of Green Mold on Polish Mushroom Farms. [J] . Szczech Magdalena, Staniaszek Miroslawa, Habdas Hanna, Vegetable Crops Research Bulletin . 2008,第Null期

机译：木霉属。 -波兰蘑菇农场出现绿色霉菌的原因。
4. Identification of Trichoderma spp. in Mushroom Farms in Serbia [C] . Dejana Kosanovi(c), Ivana Poto(c)nik, Bojan Duduk, Congress of the International Society for Mushroom Science . 2012

机译：鉴定Trichoderma SPP。在塞尔维亚的蘑菇农场
5. Etiology and epidemiology of Trichoderma harzianum rifai in association with the cultivated mushroom Agaricus bisporus (Lange) imbach. [D] . Anderson, Merrilee Gale. 2002

机译：里氏木霉与栽培蘑菇双孢蘑菇（Lange）imbach的病因和流行病学。
6. Morphological and Molecular Identification of Trichoderma Isolates on North American Mushroom Farms [O] . Alan Castle, Donna Speranzini, Nezar Rghei, 1998

机译：北美蘑菇场中木霉菌分离物的形态和分子鉴定
7. Isolation and identification of Bacillus spp. from compost material, compost and mushroom casing soil active against Trichoderma spp. [O] . Stanojević Olja, Milijašević-Marčić Svetlana, Potočnik Ivana, 2016

机译：芽孢杆菌的分离与鉴定来自堆肥材料，堆肥和蘑菇肠衣土壤对木霉菌的活性。

Identification of Trichoderma spp. in Mushroom Farms in Serbia

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