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首页> 外文期刊>Archives of Biological Sciences >ISOLATION AND IDENTIFICATION OF BACILLUS SPP. FROM COMPOST MATERIAL, COMPOST AND MUSHROOM CASING SOIL ACTIVE AGAINST TRICHODERMA SPP.
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ISOLATION AND IDENTIFICATION OF BACILLUS SPP. FROM COMPOST MATERIAL, COMPOST AND MUSHROOM CASING SOIL ACTIVE AGAINST TRICHODERMA SPP.

机译：芽孢杆菌SPP的分离和鉴定。从堆肥材料，堆肥和蘑菇套管土壤中去除毛滴虫。

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The isolation of bacteria was carried out from samples of straw and chicken manure, compost at various stages of the composting process and casing soil used for growing button mushrooms. A preliminary screening of 108 bacterial isolates for antagonistic activity against Trichoderma aggressivum f. europaeum showed that 23 tested isolates inhibited mycelial growth of the pathogenic fungus. Further screening with four indicator isolates of fungi revealed that all 23 bacterial isolates inhibited the growth of T. aggressivum f. europaeum , T. harzianum and T. koningii , while only 13 isolates inhibited the growth of T. atroviride . T. aggressivum f. europaeum proved to be the most sensitive, with many bacterial isolates generating a high percentage of growth inhibition. Only two bacterial isolates (B-129 and B-268) were successful in inhibiting the growth of all 4 tested pathogens. All 23 bacterial isolates were characterized as Gram-positive and catalase-positive and were subjected to molecular identification based on the partial sequence, the hypervariant region of the 16S rDNA. It was shown that the obtained bacterial strains belong to Bacillus subtilis , B. amyloliquefaciens , B. licheniformis and B. pumilus species.DOI: 10.2298/ABS151104073SKey words: Agaricus bisporus ; Bacillus ; biocontrol; green mold; hypervariant regionReceived: November 4, 2015; Revised: December 25, 2015; Accepted: January 21, 2016; Published online: August 9, 2016How to cite this article: Stanojevi? O, Milija?evi?-Mar?i? S, Poto?nik I, Stepanovi? M, Dimki? I, Stankovi? S, Beri? T. Isolation and identification of Bacillus spp. from compost material, compost and mushroom casing soil active against Trichoderma spp.. Arch Biol Sci. 2016;68(4):845-52.
机译：从稻草和鸡粪，堆肥过程各个阶段的堆肥样品以及用于种植蘑菇的肠衣土壤中进行细菌分离。初步筛选108种细菌菌株对侵袭性木霉f的拮抗活性。 europaeum显示，经过测试的23种分离株可抑制病原真菌的菌丝体生长。用四种真菌指示剂菌株进一步筛选显示，所有23种细菌菌株均能抑制侵略性木犀的生长。 europaeum，T. harzianum和T. koningii，而只有13株抑制T. atroviride的生长。侵略性小麦事实证明，欧洲木霉菌是最敏感的，许多细菌分离株产生很高的生长抑制百分比。只有两种细菌分离株（B-129和B-268）成功抑制了所有4种测试病原体的生长。所有23种细菌分离株均以革兰氏阳性和过氧化氢酶阳性为特征，并根据16S rDNA的高变区部分序列进行分子鉴定。结果表明，获得的细菌菌株分别为枯草芽孢杆菌，解淀粉芽孢杆菌，地衣芽孢杆菌和短小芽孢杆菌。DOI：10.2298 / ABS151104073S。芽孢杆菌;生物防治绿霉高变区接收：2015年11月4日;修订日期：2015年12月25日;接受：2016年1月21日;在线发布：2016年8月9日如何引用本文：Stanojevi？ O，Milija？evi？-Mar？i？ S，Poto？nik I，Stepanovi？ M，Dimki？我，斯坦科维？ S，Beri？ T.芽孢杆菌属的分离和鉴定。由堆肥材料，堆肥和蘑菇肠土壤组成，对木霉属菌种有活性。Arch Biol Sci。 2016; 68（4）：845-52。

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